Tartrate-resistant acid phosphate as biomarker of bone turnover over the lifespan and different physiologic stages in sheep

BMC Veterinary Research

Research Article

Nuno Dourado¹  Jorge T. Azevedo²  João P. A. Bordelo³  José A. Camassa³  Camila C. Diogo³  Carlos A. Viegas⁴  Isabel R. Dias⁴  Marília de A. Bonelli⁵ 

[1] CMEMS-UMinho, Department of Mechanical Engineering, University of Minho, Campus de Azurém, 4804-533, Guimarães, Portugal;Department of Animal Sciences, ECAV, UTAD, Vila Real, Portugal;CECAV – Centre for Animal Sciences and Veterinary Studies, UTAD, Vila Real, Portugal;Department of Veterinary Sciences, Agricultural and Veterinary Sciences School (ECAV), University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, P.O. Box 1013, 5001-801, Vila Real, Portugal;Department of Veterinary Sciences, Agricultural and Veterinary Sciences School (ECAV), University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, P.O. Box 1013, 5001-801, Vila Real, Portugal;CITAB – Centre for the Research and Technology of Agro-Environmental and Biological Sciences, UTAD, Vila Real, Portugal;Federal Rural University of Pernambuco (UFRPE), R. Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, PE, Brazil;

关键词: ALP;    Bone tissue;    Serum minerals;    Sheep;    Tartrate-resistant acid phosphatase;   

DOI  :  10.1186/s12917-017-1170-9

 received in 2016-12-09, accepted in 2017-08-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCurrently, the best resources for assessment of bone tissue using imaging techniques are expensive and available in few medical facilities, thus serum or urinary bone turnover biomarkers could be useful as early indicators of prognosis. However, there is a wide range of variability in bone turnover markers due to several factors, such as different ages and metabolic stages, thus it is important to have as much data published on the subject as possible. The aim of this study was therefore to generate a reference range for alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) and validate the already published data.ResultsSerum alkaline phosphatase decreased with age, with statistical difference between the 1 month old and the other groups and between the over 8 years and the 6 months old groups. There was also a statistical difference in the ALP levels between the 3 to 5 years old gestation and lactation groups. For serum tartrate-resistant acid phosphatase, there was a statistical difference mainly between the 1 month old and the 6 months old, 6–8 years old, and above 8 years old groups.ConclusionsThe results obtained could represent a useful tool for future studies using sheep as an animal model for orthopedic research. The different groups presented a wide variation of serum ALP and TRAP levels, however, these variations are entirely explained by known physiology. Therefore, this detailed study confirms the prediction that unexplained changes in these bone turnover markers do not occur during the lifespan of sheep.

【 授权许可】

CC BY   
© The Author(s). 2017