Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts

PeerJ

article

Lucia Melguizo-Rodríguez¹  Víctor J. Costela-Ruiz¹  Francisco J. Manzano-Moreno²  Rebeca Illescas-Montes²  Javier Ramos-Torrecillas¹  Olga García-Martínez¹  Concepción Ruiz¹ 

[1] Biomedical Group ,(BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada;Instituto Investigación Biosanitaria, ibs.Granada, University of Granada;Biomedical Group ,(BIO277), Department of Stomatology, School of Dentistry, University of Granada;Institute of Neuroscience, University of Granada

关键词: Bone tissue;    Osteoblasts;    Differentiation;    Gene expression;    NSAIDs;   

DOI  :  10.7717/peerj.5415

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundNonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used in clinical practice, which can have adverse effects on the osteoblast. The objective of this study was to determine the effect of NSAIDs on the osteoblast by analyzing the gene expression of different markers related to osteoblast maturation and function when treated in vitro with different NSAIDs.MethodsThree human osteoblast lines from bone samples of three healthy volunteers were treated with 10 µM acetaminophen, indomethacin, ketoprofen, diclofenac, ibuprofen, ketorolac, naproxen, and piroxicam. The gene expression of different markers (run related transcription factor 2 [RUNX-2], type 1 collagen [COL-I], osterix [OSX], osteocalcin [OSC], bone morphogenetic protein 2 [BMP-2] and 7 [BMP-7], transforming growth factor β1 [TGF-β1], and TGFβ receptors [TGFβR1, TGFβR2; TGFBR3]) were analyzed by real-time PCR at 24 h of treatment.ResultsExpression of RUNX-2, COL-I, OSX, was reduced by treatment with all studied NSAIDs, OSC expression was reduced by all NSAIDs except for ketoprofen, naproxen, or piroxicam. Expression of BMP-7 was reduced by all NSAIDs; BMP-2 was reduced by all except for naproxen. In general, NSAID treatment increased the expression of TGF-β1, but not of its receptors (TGFβ-R1, TGFβ-R2, andTFGβ-R3), which was either unchanged or reduced by the treatment.ConclusionThese data confirm that NSAIDs can affect osteoblast physiology, suggesting their possible impact on bone.

【 授权许可】

CC BY