Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose

Microbial Cell Factories

Research

Toshihide Yoshie¹  Chiaki Ogino¹  Akihiko Kondo¹  Satoshi Wakai²  Nanami Asai-Nakashima²  Fumiyoshi Okazaki²  Ryosuke Yamada³  Yoji Hata⁴  Hiroko Tsutsumi⁴  Hiromoto Hisada⁴ 

[1] Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, 657-8501, Kobe, Hyogo, Japan;Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, 657-8501, Kobe, Hyogo, Japan;Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, 657-8501, Kobe, Hyogo, Japan;Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan;Research Institute, Gekkeikan Sake Co. Ltd, 101 Shimotoba-koyanagi-cho, 612-8385, Fushimi-ku, Kyoto, Japan;

关键词: Aspergillus oryzae;    Kojic acid;    Cellulose;    Cellulase;    Starch;   

DOI  :  10.1186/1475-2859-13-71

 received in 2014-03-13, accepted in 2014-05-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundKojic acid (5-Hydroxy-2-(hydroxymethyl)-4-pyrone) is one of the major secondary metabolites in Aspergillus oryzae. It is widely used in food, pharmaceuticals, and cosmetics. The production cost, however, is too high for its use in many applications. Thus, an efficient and cost-effective kojic acid production process would be valuable. However, little is known about the complete set of genes for kojic acid production. Currently, kojic acid is produced from glucose. The efficient production of kojic acid using cellulose as an inexpensive substrate would help establish cost-effective kojic acid production.ResultsA kojic acid transcription factor gene over-expressing the A. oryzae strain was constructed. Three genes related to kojic acid production in this strain were transcribed in higher amounts than those found in the wild-type strain. This strain produced 26.4 g/L kojic acid from 80 g/L glucose. Furthermore, this strain was transformed with plasmid harboring 3 cellulase genes. The resultant A. oryzae strain successfully produced 0.18 g/L of kojic acid in 6 days of fermentation from the phosphoric acid swollen cellulose.ConclusionsKojic acid was produced directly from cellulose material using genetically engineered A. oryzae. Because A. oryzae has efficient protein secretion ability and secondary metabolite productivity, an A. oryzae-based cell factory could be a platform for the production of various kinds of bio-based chemicals.

【 授权许可】

Unknown   
© Yamada et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.