Cellulase and Xylanase Production from Three Isolates of Indigenous Endophytic Fungi

International Conference on Natural Products and Bioresource Science 2017

生物科学;化学

Yopi^1 ; Tasia, W.^1 ; Melliawati, R.^1

Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Cibinong, West Java, Bogor

16911, Indonesia^1

关键词: Bioprocesses;    Cellulase;    Cellulase and xylanase;    Endophytic fungi;    Macroscopic and microscopic;    Madagascar periwinkles;    Traditional medicinal plants;    Xylanases;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/101/1/012035/pdf
DOI  :  10.1088/1755-1315/101/1/012035

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Cellulases and hemicellulases have good potential to be used in energy production, in pulp, paper, textile industries, as well as in animal feed industries. Moreover, its utilization in food industries also cannot be ignored, among others, cellulase and xylanase roles in bakery, wine, and fruit and vegetables juice production. One of the potential enzyme source is endophytic fungi. Object of this study is to explore the potency of endophytic fungi isolated from medicinal plants as source of cellulolytic and xylanolytic enzymes. HL.47F.216 is endophytic fungi isolated from traditional medicinal plants ironwood tree was determined as xylanase producer. HL.51F.235 from pin-flower tree is cellulase producer, while CBN.6F.29 which produces both xylanase and cellulase is originated from Madagascar periwinkle. HL.47F.216 showed 2.5 cm in clear zone diameter and its xylanase activity was 0.262 U/mL with optimum condition pH 7 at 50°C. HL.51F.235 showed 2.4 cm clear zone diameter and 0.239 U/mL of cellulase activity at pH 5 and 70°C. CBN.6F.29 showed 2.8 cm and 0.394 U/mL (pH 5, 40°C) for its cellulase activity, while 2.3 cm and 0.439 U/mL (pH 8, 70°C) for its xylanase activity. Xylanase from HL.47F.216 and CBN.6F.29 showed low molecular masses of 20 kDa and 37-50 kDa, respectively. Molecular masses for cellulases from HL.51F.235 and CBN.6F.29 were 25 and 50 kDa for HL.51F.235 and 100 kDa for CBN.6F.29. Based on macroscopic and microscopic identification, fungal isolate CBN.6F.29 is a member of Class Coelomycetes, while HL.47F.216 was Acremonium sp. and HL.51F.235 was Aspergillus nigri.