Spheroid formation of human thyroid cancer cells under simulated microgravity: a possible role of CTGF and CAV1

Cell Communication and Signaling

Research

Markus Wehland¹  Jessica Pietsch¹  Elisabeth Warnke¹  Manfred Infanger¹  Daniela Grimm²  Mark Görög³  Ruth Hemmersbach³  Markus Braun⁴  Jayashree Sahana⁵  Xiao Ma⁵  Johann Bauer⁶ 

[1] Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany;Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany;Institute of Biomedicine, Pharmacology, Aarhus University, Wilhelm Meyers Allé 4, DK-8000 Aarhus C, Denmark;DLR, German Aerospace Center, Institute of Aerospace Medicine, Cologne, Germany;Institute for Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Gravitational Biology Group, Kirschallee 1, 53115, Bonn, Germany;Institute of Biomedicine, Pharmacology, Aarhus University, Wilhelm Meyers Allé 4, DK-8000 Aarhus C, Denmark;Max-Planck Institute of Biochemistry, Martinsried, Germany;

关键词: Adherent growth;    Three-dimensional growth;    Cytokine;    Microgravity;    MCTS;    RPM;    2D clinostat;   

DOI  :  10.1186/1478-811X-12-32

 received in 2014-01-24, accepted in 2014-04-28,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMulticellular tumor spheroids (MCTS) formed scaffold-free under microgravity are of high interest for research and medicine. Their formation mechanism can be studied in space in real microgravity or on Earth using ground-based facilities (GBF), which simulate microgravity. On Earth, these experiments are more cost-efficient and easily performable. However, each GBF might exert device-specific and altered superimposingly gravity-dependent effects on the cells.ResultsFTC-133 human thyroid cancer cells were cultivated on a 2D clinostat (CN) and a random positioning machine (RPM) and compared with corresponding 1 g control cells. Harvested cell samples were investigated by microscopy, quantitative realtime-PCR and Multi-Analyte Profiling. Spheroid formation and growth occurred during 72 h of cultivation on both devices. Cytokine secretion and gene activation patterns frequently altered in different ways, when the cells were cultured either on the RPM or the CN. A decreased expression of CAV1 and CTGF in MCTS compared to adherent cells was observed after cultivation on both machines.ConclusionThe development of MCTS proceeds similarly on the RPM and the CN resembling the situation observed under real microgravity conditions, while no MCTS formation was observed at 1 g under identical experimental conditions. Simultaneously, changes in the regulation of CTGF and CAV1 appeared in a comparable manner on both machines. A relationship between these molecules and MCTS formation is discussed.

【 授权许可】

Unknown   
© Warnke et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.