期刊论文详细信息
Bone Reports
Thirty days of spaceflight does not alter murine calvariae structure despite increased Sost expression
Brandon R. Macias1  Alan R. Hargens2  Timothy R. Macaulay3  Jamila H. Siamwala3 
[1] KBRwyle, 2400 NASA Parkway, Houston, TX 77058, USA;University of California, San Diego, Altman Clinical and Translational Research Institute, Lower Level 2 West 417, 9452 Medical Center Drive, La Jolla, CA 92037, USA;University of California, San Diego, UCSD Medical Center, Orthopaedic Surgery Department, 350 Dickinson Street, Suite 121, Mail Code 8894, San Diego, CA 92103-8894, USA;
关键词: Microgravity;    Mechanotransduction;    Hydrostatic pressure;    Osteocyte;    Weightlessness;    Skull;   
DOI  :  10.1016/j.bonr.2017.08.004
来源: DOAJ
【 摘 要 】

Previously our laboratory documented increases in calvaria bone volume and thickness in mice exposed to 15 days of spaceflight aboard the NASA Shuttle mission STS-131. However, the tissues were not processed for gene expression studies to determine what bone formation pathways might contribute to these structural adaptations. Therefore, this study was designed to investigate both the structural and molecular changes in mice calvariae after a longer duration of spaceflight. The primary purpose was to determine the calvaria bone volume and thickness of mice exposed to 30 days of spaceflight using micro-computed tomography for comparison with our previous findings. Because sclerostin, the secreted glycoprotein of the Sost gene, is a potent inhibitor of bone formation, our second aim was to quantify Sost mRNA expression using quantitative PCR. Calvariae were obtained from six mice aboard the Russian 30-day Bion-M1 biosatellite and seven ground controls. In mice exposed to 30 days of spaceflight, calvaria bone structure was not significantly different from that of their controls (bone volume was about 5% lower in spaceflight mice, p = 0.534). However, Sost mRNA expression was 16-fold (16.4 ± 0.4, p < 0.001) greater in the spaceflight group than that in the ground control group. Therefore, bone formation may have been suppressed in mice exposed to 30 days of spaceflight. Genetic responsiveness (e.g. sex or strain of animals) or in-flight environmental conditions other than microgravity (e.g. pCO2 levels) may have elicited different bone adaptations in STS-131 and Bion-M1 mice. Although structural results were not significant, this study provides biochemical evidence that calvaria mechanotransduction pathways may be altered during spaceflight, which could reflect vascular and interstitial fluid adaptations in non-weight bearing bones. Future studies are warranted to elucidate the processes that mediate these effects and the factors responsible for discordant calvaria bone adaptations between STS-131 and Bion-M1 mice.

【 授权许可】

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