Developmental expression of p97/VCP (Valosin-containing protein) and Jab1/CSN5 in the rat testis and epididymis

Reproductive Biology and Endocrinology

Research

Ufuk Tas¹  Seda Ocakli²  Huseyin Aslan²  Sevil Cayli²  Zafer Karaca²  Fikret Erdemir³  Tamer Yener⁴ 

[1] Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey;Department of Histology and Embryology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey;Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey;Experimental Animal Center, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey;

关键词: Sertoli Cell;    Seminiferous Tubule;    Ubiquitin Proteasome System;    Round Spermatid;    Seminiferous Epithelium;   

DOI  :  10.1186/1477-7827-9-117

 received in 2011-05-27, accepted in 2011-08-19,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe ubiquitin proteasome system (UPS) is a key player in regulating many cellular processes via proteasomal degradation of ubiquitinated proteins. Recently published data show that Jab1/CSN5 interacts with p97/VCP and controls the ubiquitination status of proteins bound to p97/VCP in mouse and human cells. However, coexpression of p97/VCP and Jab1/CSN5 in the developing rat testis and epididymis has not previously been studied.MethodsTesticular and epididymal tissues from 5-, 15-, 30-, and 60-day-old rats were examined by immunohistochemistry and Western blotting. Colocalisation of proteins was determined by immunofluorescence microscopy.ResultsIn the 5-day-old rat testis, p97/VCP and Jab1/CSN5 were specifically expressed in gonocytes. The expression of p97/VCP and Jab1/CSN5 significantly increased at day 15 and was found in spermatogonia, Sertoli cells and spermatocytes. In 30- and 60-day-old rat testes, p97/VCP indicated moderate to strong expression in Sertoli cells, spermatogonia, round and elongating spermatids. However, moderate to weak expression was observed in spermatocytes. Jab1/CSN5 showed strong expression in spermatogonia and spermatocytes, while relatively moderate expression was observed in round and elongating spermatids in 30- and 60-day-old rat testes. In contrast, in the epididymis, the expression of both proteins gradually increased from 5 to 60 days of age. After rats reached 2 weeks of age, the expression of both proteins was mostly restricted to the basal and principal cells of the caput epididymis.ConclusionsOur study suggests that p97/VCP and Jab1/CSN5 could be an important part of the UPS in the developing rat testis and epididymis and that both proteins may be involved in the regulation of spermatogenesis and epididymal epithelial functions.

【 授权许可】

CC BY   
© Cayli et al; licensee BioMed Central Ltd. 2011