Cadmium suppresses the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis and aberrant ultrastructure

Reproductive Biology and Endocrinology

Research

Yin Lu¹  Li Zhu¹  Sijun Deng¹  Hui Yuan¹  Chengzhi Guo¹  Lixin Wen¹  Liyun Yuan¹  Jing Wu¹  Ming Zhang²  Zuping He³ 

[1] College of Veterinary Medicine, Hunan Agricultural University, 410128, Changsha, Hunan, P. R. China;College of Veterinary Medicine, Hunan Agricultural University, 410128, Changsha, Hunan, P. R. China;Jiangxi Biotech Vocational College, 330200, Nanchang, Jiangxi, P. R. China;Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Medical Center, 3900 Reservoir Road NW, 20057, Washington, DC, USA;

关键词: Cadmium;    Sertoli Cell;    Seminiferous Tubule;    Male Germ Cell;    Spermatogonial Stem Cell;   

DOI  :  10.1186/1477-7827-8-97

 received in 2010-04-10, accepted in 2010-08-16,  发布年份 2010

来源: Springer

【 摘 要 】

ObjectiveVery little information is known about the toxic effects of cadmium on somatic cells in mammalian testis. The objective of this study is to explore the toxicity of cadmium on piglet Sertoli cells.MethodsSertoli cells were isolated from piglet testes using a two-step enzyme digestion and followed by differential plating. Piglet Sertoli cells were identified by oil red O staining and Fas ligand (FasL) expression as assayed by immunocytochemistry and expression of transferrin and androgen binding protein by RT-PCR. Sertoli cells were cultured in DMEM/F12 supplemented with 10% fetal calf serum in the absence or presence of various concentrations of cadmium chloride, or treatment with p38 MAPK inhibitor SB202190 and with cadmium chloride exposure. Apoptotic cells in seminiferous tubules of piglets were also performed using TUNEL assay in vivo.ResultsCadmium chloride inhibited the proliferation of Piglet Sertoli cells as shown by MTT assay, and it increased malondialdehyde (MDA) but reduced superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) activity. Inhibitor SB202190 alleviated the proliferation inhibition of cadmium on piglet Sertoli cells. Comet assay revealed that cadmium chloride caused DNA damage of Piglet Sertoli cells and resulted in cell apoptosis as assayed by flow cytometry. The in vivo study confirmed that cadmium induced cell apoptosis in seminiferous tubules of piglets. Transmission electronic microscopy showed abnormal and apoptotic ultrastructure in Piglet Sertoli cells treated with cadmium chloride compared to the control.Conclusioncadmium has obvious adverse effects on the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis, and aberrant morphology. This study thus offers novel insights into the toxicology of cadmium on male reproduction.

【 授权许可】

Unknown   
© Zhang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.