Effects of various heavy metal nanoparticles on Enterococcus hirae and Escherichia coli growth and proton-coupled membrane transport

Journal of Nanobiotechnology

Research

Vladimir Gevorkyan¹  Hrachik Vardapetyan²  Zaruhi Vardanyan³  Armen Trchounian⁴  Michail Ananyan⁵ 

[1] Department of Materials Technology and Structures of Electronic Technique, Institute of Mathematics and High Technologies, Russian-Armenian (Slavonic) University, 123 H. Emin Str., 0051, Yerevan, Armenia;Department of Medicinal Biochemistry and Biotechnology, Institute of Mathematics and High Technologies, Russian-Armenian (Slavonic) University, 123 H. Emin Str., 0051, Yerevan, Armenia;Research Institute of Biology, Faculty of Biology, Yerevan State University, 1 A. Manoukian Str., 0025, Yerevan, Armenia;Research Institute of Biology, Faculty of Biology, Yerevan State University, 1 A. Manoukian Str., 0025, Yerevan, Armenia;Department of Medicinal Biochemistry and Biotechnology, Institute of Mathematics and High Technologies, Russian-Armenian (Slavonic) University, 123 H. Emin Str., 0051, Yerevan, Armenia;Department of Microbiology, Microbes and Plants Biotechnology, Faculty of Biology, Yerevan State University, 1 A. Manoukian Str., 0025, Yerevan, Armenia;“Nano-industry” Concern, 4 Bardin Str., 1 bulk, 119334, Moscow, Russia;

关键词: Nanoparticles;    Heavy metals;    Enterococcus hirae;    Escherichia coli;    Bacterial growth;    The FF-ATPase;   

DOI  :  10.1186/s12951-015-0131-3

 received in 2015-07-24, accepted in 2015-10-06,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundDue to bacterial resistance to antibiotics there is a need for new antimicrobial agents. In this respect nanoparticles can be used as they have expressed antibacterial activity simultaneously being more reactive compared to their bulk material. The action of zinc (II), titanium (IV), copper (II) and (I) oxides thin films with nanostructured surface and silver nanoscale particles on Enterococcus hirae and Escherichia coli growth and membrane activity was studied by using microbiological, potentiometric and spectrophotometric methods.ResultsIt was revealed that sapphire base plates with deposited ZnO, TiO2, CuO and Cu2O nanoparticles had no effects neither on E. hirae nor E. coli growth both on agar plates and in liquid medium. Concentrated Ag nanoparticles colloid solution markedly affected bacterial growth which was expressed by changing growth properties. E. hirae was able to grow only at <1:200 dilutions of Ag nanoparticles while E. coli grew even at 1:10 dilution. At the same time Ag nanoparticles directly affected membranes, as the FOF1-ATPase activity and H+-coupled transport was changed either (E. coli were less susceptible to nanoparticles compared to E. hirae). Ag nanoparticles increased H+ and K+ transport even in the presence of N,N′-dicyclohexylcarbodiimide (DCCD), inhibitor of FOF1. The stoichiometry of DCCD-inhibited ion fluxes was disturbed.ConclusionsThese results point out to distinguishing antibacterial effects of Ag nanoparticles on different bacteria; the difference between effects can be explained by peculiarities in bacterial membrane structure and properties. H+-K+-exchange disturbance by Ag nanoparticles might be involved in antibacterial effects on E. hirae. The role of FOF1 in antibacterial action of Ag nanoparticles was shown using atpD mutant lacked β subunit in F1.

【 授权许可】

CC BY   
© Vardanyan et al. 2015