Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice

Lipids in Health and Disease

Research

Si-Cong Ma¹  Xiao-Lin Wang²  Xiao-Bo Li³  Zheng-Yang Wang³  Jing-Yi Zhang⁴  Chun-Min Li⁴  Yi-Min Mao⁴  Ya-Nan Ming⁴  Xiao-Lin Liu⁵ 

[1] Department of Interventional Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China;Department of Pharmacology, School of Medicine, Shanghai Jiao Tong University, Institute of Medical Sciences, Shanghai, China;Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, 200032, Shanghai, China;Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai, China;Division of Gastroenterology and Hepatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China;

关键词: Acetaminophen;    Phosphatidylcholine;    Phosphatidylethanolamine;    Lipidomics;    Liver injury;   

DOI  :  10.1186/s12944-017-0540-4

 received in 2017-02-23, accepted in 2017-08-02,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAcetaminophen (APAP) overdose is one of the most common causes of acute liver failure in many countries. The aim of the study was to describe the profiling of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in the plasma and liver of Acetaminophen -induced liver injured mice.MethodsA time course study was carried out using C57BL/6 mice after intraperitoneal administration of 300 mg/kg Acetaminophen 1 h, 3 h, 6 h, 12 h and 24 h. A high-throughput liquid chromatography mass spectrometry (LC-MS) lipidomic method was utilized to detect phosphatidylcholine and phosphatidylethanolamine species in the plasma and liver. The expressions of phosphatidylcholine and phosphatidylethanolamine metabolism related genes in liver were detected by quantitative Reverse transcription polymerase chain reaction (qRT-PCR) and Western-blot.ResultsFollowing Acetaminophen treatment, the content of many PC and PE species in plasma increased from 1 h time point, peaked at 3 h or 6 h, and tended to return to baseline at 24 h time point. The relative contents of almost all PC species in liver decreased from 1 h, appeared to be lowest at 6 h, and then return to normality at 24 h, which might be partly explained by the suppression of phospholipases mRNA expressions and the induction of choline kinase (Chka) expression. Inconsistent with PC profile, the relative contents of many PE species in liver increased upon Acetaminophen treatment, which might be caused by the down-regulation of phosphatidylethanolamine N-methyltransferase (Pemt).ConclusionsAcetaminophen overdose induced dramatic change of many PC and PE species in plasma and liver, which might be caused by damaging hepatocytes and interfering the phospholipid metabolism in Acetaminophen -injured liver.

【 授权许可】

CC BY   
© The Author(s). 2017