Forced expiration measurements in mouse models of obstructive and restrictive lung diseases

Respiratory Research

Research

Lore Pollaris¹  Peter HM Hoet¹  Jeroen AJ Vanoirbeek¹  Fien C. Devos¹  Benoit Nemery¹  Sven Seys²  André Maaske³  Matthias Tenbusch⁴  Annette Robichaud⁵  Rik Lories⁶  Carolina Aznar Lopez⁶  Erik Verbeken⁷ 

[1] Center for Environment and Health, KU Leuven, Leuven, Belgium;Clinical Immunology, KU Leuven, Leuven, Belgium;Molecular and Medical Virology, Ruhr-University, Bochum, Germany;Molecular and Medical Virology, Ruhr-University, Bochum, Germany;Institute of Clinical and Molecular Virology, University Hospital Erlangen, Erlangen-Nürnberg, Germany;SCIREQ Scientific Respiratory Equipment Inc., Montreal, QC, Canada;Skeletal Biology and Engineering Research Center, KU Leuven, Leuven, Belgium;Translational Cell and Tissue Research, KU Leuven, Leuven, Belgium;

关键词: Fibrosis;    Emphysema;    Asthma;    Acute lung injury;    Mice;    Forced oscillations technique;    Negative pressure forced expiration;    Airway resistance;    Tissue elastance;    Forced expiratory volume;    Forced vital capacity;   

DOI  :  10.1186/s12931-017-0610-1

 received in 2017-02-13, accepted in 2017-06-12,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPulmonary function measurements are important when studying respiratory disease models. Both resistance and compliance have been used to assess lung function in mice. Yet, it is not always clear how these parameters relate to forced expiration (FE)-related parameters, most commonly used in humans. We aimed to characterize FE measurements in four well-established mouse models of lung diseases.MethodDetailed respiratory mechanics and FE measurements were assessed concurrently in Balb/c mice, using the forced oscillation and negative pressure-driven forced expiration techniques, respectively. Measurements were performed at baseline and following increasing methacholine challenges in control Balb/c mice as well as in four disease models: bleomycin-induced fibrosis, elastase-induced emphysema, LPS-induced acute lung injury and house dust mite-induced asthma.ResultsRespiratory mechanics parameters (airway resistance, tissue damping and tissue elastance) confirmed disease-specific phenotypes either at baseline or following methacholine challenge. Similarly, lung function defects could be detected in each disease model by at least one FE-related parameter (FEV0.1, FEF0.1, FVC, FEV0.1/FVC ratio and PEF) at baseline or during the methacholine provocation assay.ConclusionsFE-derived outcomes in four mouse disease models behaved similarly to changes found in human spirometry. Routine combined lung function assessments could increase the translational utility of mouse models.

【 授权许可】

CC BY   
© The Author(s). 2017