The main e-cigarette component vegetable glycerin enhances neutrophil migration and fibrosis in endotoxin-induced lung injury via p38 MAPK activation

Respiratory Research

Research

Hao Chen¹  Huai-Hsuan Wu¹  Wen-Kuang Yu²  Wei-Chih Chen²  Kuang-Yao Yang³  Vincent Yi-Fong Su⁴ 

[1] Department of Chest Medicine, Taipei Veterans General Hospital, No. 201, Sec. 2, Shi-Pai Road, 11217, Taipei, Taiwan;Faculty of Medicine, School of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec 2, Linong St, 11221, Taipei, Taiwan;Department of Chest Medicine, Taipei Veterans General Hospital, No. 201, Sec. 2, Shi-Pai Road, 11217, Taipei, Taiwan;Faculty of Medicine, School of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec 2, Linong St, 11221, Taipei, Taiwan;Department of Chest Medicine, Taipei Veterans General Hospital, No. 201, Sec. 2, Shi-Pai Road, 11217, Taipei, Taiwan;Institute of Emergency and Critical Care Medicine, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan;Cancer Progression Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan;Faculty of Medicine, School of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec 2, Linong St, 11221, Taipei, Taiwan;Department of Internal Medicine, Taipei City Hospital, Taipei City Government, Taipei, Taiwan;Department of Exercise and Health Sciences, College of Kinesiology, University of Taipei, Taipei, Taiwan;

关键词: e-cigarette;    Chemotaxis;    Fibrosis;    p38 MAPK;    Acute lung injury;    Vegetable glycerin;   

DOI  :  10.1186/s12931-022-02307-z

 received in 2022-09-22, accepted in 2022-12-27,  发布年份 2022

来源: Springer

【 摘 要 】

We investigated the effects of vegetable glycerin (VG), a main e-cigarette constituent, on endotoxin-induced acute lung injury (ALI). Mice received intratracheal administration of 30% VG in phosphate buffered saline (PBS) vehicle or only PBS (control) for 4 days. On Day 5, mice received an intratracheal instillation of lipopolysaccharide (LPS) (LPS group and VG + LPS group) or PBS (VG group and control group).Lung histopathology, expression of chemokine receptors, and regulatory signaling were analyzed 24 h after the Day 5 treatment. VG significantly increased ALI-associated histopathological and fibrotic changes in both the VG group and LPS-induced ALI mice (VG + LPS group). Immunohistochemistry (IHC) and western blot analyses revealed that VG administration resulted in upregulation of neutrophil markers [lymphocyte antigen 6 complex locus G6D (Ly6G) and myeloperoxidase (MPO)] as well as upregulation of the expression of transforming growth factor-β (TGF-β), a central mediator of fibrogenesis, in the lungs of both VG and VG + LPS groups. VG enhanced the expression of adhesion molecules [very late antigen 4 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1)] and increased activation of p38 mitogen-activated protein kinase (p38 MAPK) to prompt neutrophil recruitment in the lungs of mice with ALI. Intraperitoneal administration of a p38 inhibitor attenuated these histopathological changes significantly as well as VG-induced upregulation in expression of Ly6G, MPO, VLA-4, VCAM-1, TGF-β, and collagen-1 in mice with ALI. In conclusion, VG enhances neutrophil chemotaxis and fibrosis and it amplifies the inflammatory response associated with LPS-induced ALI in the lungs via enhancement of p38 MAPK activity.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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Fig. 1

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