Comparative transcriptome analysis of high- and low-embryogenic Hevea brasiliensis genotypes reveals involvement of phytohormones in somatic embryogenesis

BMC Plant Biology

Research

Ling Li¹  Yanfen Qiu¹  Xiaolong Sun¹  Guoping Liang¹  Min Tang¹  Hai Tian¹  Wencai Yu¹  Mingchun Gui¹ 

[1] The Center of Rubber Research, Yunnan Institute of Tropical Crops, Xishuangbanna, China;

关键词: Abscisic acid;    Auxins;    Cytokinins;    Ethylene;    HO;    MAPK signaling;    Somatic embryogenesis;   

DOI  :  10.1186/s12870-023-04432-3

 received in 2023-05-30, accepted in 2023-08-31,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundRubber plant (Hevea brasiliensis) is one of the major sources of latex. Somatic embryogenesis (SE) is a promising alterative to its propagation by grafting and seed. Phytohormones have been shown to influence SE in different plant species. However, limited knowledge is available on the role of phytohormones in SE in Hevea. The anther cultures of two Hevea genotypes (Yunyan 73477-YT and Reken 628-RT) with contrasting SE rate were established and four stages i.e., anthers (h), anther induced callus (y), callus differentiation state (f), and somatic embryos (p) were studied. UPLC-ESI-MS/MS and transcriptome analyses were used to study phytohormone accumulation and related expression changes in biosynthesis and signaling genes.ResultsYT showed higher callus induction rate than RT. Of the two genotypes, only YT exhibited successful SE. Auxins, cytokinins (CKs), abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), gibberellins (GAs), and ethylene (ETH) were detected in the two genotypes. Indole-3-acetic acid (IAA), CKs, ABA, and ETH had notable differences in the studied stages of the two genotypes. The differentially expressed genes identified in treatment comparisons were majorly enriched in MAPK and phytohormone signaling, biosynthesis of secondary metabolites, and metabolic pathways. The expression changes in IAA, CK, ABA, and ETH biosynthesis and signaling genes confirmed the differential accumulation of respective phytohormones in the two genotypes.ConclusionThese results suggest potential roles of phytohormones in SE in Hevea.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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