Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

Plant Methods

Sergi Munné-Bosch¹  Maren Müller¹ 

[1] Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal, 645, E-08028 Barcelona, Spain

关键词: Rosmarinus officinalis;    1-amino-cyclopropane-1-carboxyic acid;    Jasmonic acid;    Salicylic acid;    Gibberellins;    Cytokinins;    Abscisic acid;    Auxins;    Phytohormones;    UPLC/ESI-MS/MS;   

Others  :  828716
DOI  :  10.1186/1746-4811-7-37

 received in 2011-07-12, accepted in 2011-11-18,  发布年份 2011

【 摘 要 】

Background

Plant hormones play a pivotal role in several physiological processes during a plant's life cycle, from germination to senescence, and the determination of endogenous concentrations of hormones is essential to elucidate the role of a particular hormone in any physiological process. Availability of a sensitive and rapid method to quantify multiple classes of hormones simultaneously will greatly facilitate the investigation of signaling networks in controlling specific developmental pathways and physiological responses. Due to the presence of hormones at very low concentrations in plant tissues (10^-9 M to 10^-6 M) and their different chemistries, the development of a high-throughput and comprehensive method for the determination of hormones is challenging.

Results

The present work reports a rapid, specific and sensitive method using ultrahigh-performance liquid chromatography coupled to electrospray ionization tandem spectrometry (UPLC/ESI-MS/MS) to analyze quantitatively the major hormones found in plant tissues within six minutes, including auxins, cytokinins, gibberellins, abscisic acid, 1-amino-cyclopropane-1-carboxyic acid (the ethylene precursor), jasmonic acid and salicylic acid. Sample preparation, extraction procedures and UPLC-MS/MS conditions were optimized for the determination of all plant hormones and are summarized in a schematic extraction diagram for the analysis of small amounts of plant material without time-consuming additional steps such as purification, sample drying or re-suspension.

Conclusions

This new method is applicable to the analysis of dynamic changes in endogenous concentrations of hormones to study plant developmental processes or plant responses to biotic and abiotic stresses in complex tissues. An example is shown in which a hormone profiling is obtained from leaves of plants exposed to salt stress in the aromatic plant, Rosmarinus officinalis.

【 授权许可】

   
2011 Müller and Munné-Bosch; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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