Anti-inflammatory effects of methoxyphenolic compounds on human airway cells

Journal of Inflammation

Research

David K Johnson¹  Kenneth R Houser²  Faoud T Ishmael² 

[1] Department of Chemistry, State University of New York College at Geneseo, 14454, Geneseo, NY, USA;Departments of Medicine and Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, 17033, Hershey, PA, USA;

关键词: Cytokines;    Inflammation;    Airway;    Epithelium;    Methoxyphenols;    Post-transcriptional regulation;   

DOI  :  10.1186/1476-9255-9-6

 received in 2011-10-03, accepted in 2012-03-13,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe respiratory epithelium plays a central role in the inflammatory response in asthma and other diseases. Methoxyphenolic compounds are purported to be effective anti-inflammatory agents, but their effects on the airway epithelium have not been well characterized.MethodsHuman airway cells were stimulated with TNF-α in the presence or absence of 4-substituted methoxyphenols and resveratrol. The expression of various cytokines was measured by qPCR, ELISAs, and protein arrays. Reactive oxygen species (ROS) production was measured with a reactive fluorescent probe (3',6'-diacetate-2',7'-dichlorofluorescein). Activation of NF-κB was measured by nuclear translocation and phosphorylation. Ribonuclear protein association with mRNA was assessed with a biotin-RNA affinity isolation assay.ResultsMultiple inflammatory mediators were inhibited by methoxyphenols, including: CCL2, CCL5, IL-6, IL-8, ICAM-1, MIF, CXCL1, CXCL10, and Serpin E1. IC50 values were obtained for each compound that showed significant anti-inflammatory activity: diapocynin (20.3 μM), resveratrol (42.7 μM), 2-methoxyhydroquinone (64.3 μM), apocynin (146.6 μM), and 4-amino-2-methoxyphenol (410 μM). The anti-inflammatory activity did not correlate with inhibition of reactive oxygen species production or NF-κB activation. However, methoxyphenols inhibited binding of the RNA-binding protein HuR to mRNA, indicating that they may act post-transcriptionally.ConclusionsMethoxyphenols demonstrate anti-inflammatory activity in human airway cells. More potent compounds that act via similar mechanisms may have therapeutic potential as novel anti-inflammatory agents.

【 授权许可】

Unknown   
© Houser et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.