Water deficit mechanisms in perennial shrubs Cerasus humilis leaves revealed by physiological and proteomic analyses

Proteome Science

Research

Jing Ren¹  Lijuan Zhou²  Lina Sun²  Jiewan Wang²  Xingshun Song³  Zepeng Yin⁴  Yulong Liu⁵ 

[1] College of Food Science; Key Laboratory of Dairy Science, Ministry of Education, Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People’s Republic of China;Department of Genetics, College of Life Science, Northeast Forestry University, 150040, Harbin, People’s Republic of China;Department of Genetics, College of Life Science, Northeast Forestry University, 150040, Harbin, People’s Republic of China;State Key Laboratory of Tree Genetic sand Breeding, Northeast Forestry University, 150040, Harbin, People’s Republic of China;Department of Genetics, College of Life Science, Northeast Forestry University, 150040, Harbin, People’s Republic of China;State Key Laboratory of Tree Genetic sand Breeding, Northeast Forestry University, 150040, Harbin, People’s Republic of China;Horticulture Department, College of Horticulture, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, 110866, Shenyang, People’s Republic of China;Forest Engineering and Environment Research Institute of Heilongjiang Province, No. 134 Haping Road, Nangang District, 150081, Harbin, Heilongjiang, People’s Republic of China;

关键词: Cerasus humilis;    Proteomics;    ROS;    Water deficit;    Perennial shrubs;    qRT-PCR;   

DOI  :  10.1186/s12953-017-0117-1

 received in 2016-11-23, accepted in 2017-04-26,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundDrought (Water deficit, WD) poses a serious threat to extensively economic losses of trees throughout the world. Chinese dwarf cherry (Cerasus humilis) is a good perennial plant for studying the physiological and sophisticated molecular network under WD. The aim of this study is to identify the effect of WD on C. humilis through physiological and global proteomics analysis and improve understanding of the WD resistance of plants.MethodsCurrently, physiological parameters were applied to investigate C. humilis response to WD. Moreover, we used two-dimensional gel electrophoresis (2DE) to identify differentially expressed proteins in C. humilis leaves subjected to WD (24 d). Furthermore, we also examined the correlation between protein and transcript levels.ResultsSeveral physiological parameters, including relative water content and Pn were reduced by WD. In addition, the malondialdehyde (MDA), relative electrolyte leakage (REL), total soluble sugar, and proline were increased in WD-treated C. humilis. Comparative proteomic analysis revealed 46 protein spots (representing 43 unique proteins) differentially expressed in C. humilis leaves under WD. These proteins were mainly involved in photosynthesis, ROS scavenging, carbohydrate metabolism, transcription, protein synthesis, protein processing, and nitrogen and amino acid metabolisms, respectively.ConclusionsWD promoted the CO2 assimilation by increase light reaction and Calvin cycle, leading to the reprogramming of carbon metabolism. Moreover, the accumulation of osmolytes (i.e., proline and total soluble sugar) and enhancement of ascorbate-glutathione cycle and glutathione peroxidase/glutathione s-transferase pathway in leaves could minimize oxidative damage of membrane and other molecules under WD. Importantly, the regulation role of carbohydrate metabolisms (e. g. glycolysis, pentose phosphate pathways, and TCA) was enhanced. These findings provide key candidate proteins for genetic improvement of perennial plants metabolism under WD.

【 授权许可】

CC BY   
© The Author(s). 2017