Lipids containing medium-chain fatty acids are specific to post-whole genome duplication Saccharomycotina yeasts

BMC Evolutionary Biology

Research Article

Thierry Chardot¹  Michel Canonge¹  Bernard Cintrat¹  Marie Pouteaux¹  Marine Froissard¹  Serge Casaregola²  Sabrina Mohand-Oumoussa²  Noémie Jacques²  Stéphane E. Guillouet³ 

[1] INRA, UMR 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, 78026, Versailles cedex, France;AgroParisTech, UMR 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, 78026, Versailles cedex, France;INRA, UMR 1319, Institut MICALIS, CIRM-Levures, 78850, Thiverval-Grignon, France;AgroParisTech, UMR 1319, Institut MICALIS, CIRM-Levures, 78850, Thiverval-Grignon, France;Université de Toulouse, INSA, UPS, INP, LISBP, 135 Av. de Rangueil, 31077, Toulouse, France;

关键词: Lipid;    Medium-chain fatty acids;    Saccharomycotina;    Yeasts;    Phylogeny;   

DOI  :  10.1186/s12862-015-0369-2

 received in 2014-08-14, accepted in 2015-04-29,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundYeasts belonging to the subphylum Saccharomycotina have been used for centuries in food processing and, more recently, biotechnology. Over the past few decades, these yeasts have also been studied in the interest of their potential to produce oil to replace fossil resources. Developing yeasts for massive oil production requires increasing yield and modifying the profiles of the fatty acids contained in the oil to satisfy specific technical requirements. For example, derivatives of medium-chain fatty acids (MCFAs, containing 6–14 carbons) are used for the production of biodiesels, cleaning products, lubricants and cosmetics. Few studies are available in the literature on the production of MCFAs in yeasts.ResultsWe analyzed the MCFA content in Saccharomyces cerevisiae grown in various conditions. The results revealed that MCFAs preferentially accumulated when cells were grown on synthetic media with a high C/N ratio at low temperature (23 °C). Upon screening deletion mutant strains for genes encoding lipid droplet-associated proteins, we found two genes, LOA1 and TGL3, involved in MCFA homeostasis. A phylogenetic analysis on 16 Saccharomycotina species showed that fatty acid profiles differed drastically among yeasts. Interestingly, MCFAs are only present in post-whole genome duplication yeast species.ConclusionsIn this study, we produced original data on fatty acid diversity in yeasts. We demonstrated that yeasts are amenable to genetic and metabolic engineering to increase their MCFA production. Furthermore, we revealed that yeast lipid biodiversity has not been fully explored, but that yeasts likely harbor as-yet-undiscovered strains or enzymes that can contribute to the production of high-value fatty acids for green chemistry.

【 授权许可】

CC BY   
© Froissard et al.; licensee BioMed Central. 2015