Analysis of a signal transduction pathway involved in leaf epidermis differentiation.

Becraft, Philip W.

Iowa State University

关键词: Epidermis;    59 Basic Biological Sciences;    Proteins;    Amino Acid Sequence;    Yeasts;   

DOI  :  10.2172/840253
RP-ID  :  NONE
RP-ID  :  FG02-98ER20303
RP-ID  :  840253

美国|英语

来源: UNT Digital Library

【 摘 要 】

The major objective of this study was to identify and analyze signal transduction factors that function with the CR4 receptor kinase. We pursued this analysis in Arabidopsis. Analysis of other members of the ACR4 related receptor (CRR) family produced biochemical evidence consistent with some of them functioning in ACR4 signal transduction. Yeast 2-hybrid identified six proteins that interact with the cytoplasmic domain of ACR4, representing putative downstream signal transduction components. The interactions for all 6 proteins were verified by in vitro pull down assays. Five of the interacting proteins were phosphorylated by ACR4. We also identified candidate interactors with the extracellular TNFR domain. We hypothesize this may be the ligand binding domain for ACR4. In one approach, yeast 2-hybrid was again used and five candidate proteins identified. Nine additional candidates were identified in a genome wide scan of Arabidopsis amino acid sequences that threaded onto the TNF structure.