DNA Compaction by Yeast Mitochondrial Protein ABF2p

Friddle, R W ; Klare, J E ; Noy, A ; Corzett, M ; Balhorn, R ; Baskin, R J ; Martin, S S ; Baldwin, E P

Lawrence Livermore National Laboratory

关键词: Dna;    Yeasts;    Polymers;    Statistical Mechanics;    59 Basic Biological Sciences;   

DOI  :  10.2172/15007313
RP-ID  :  UCRL-ID-153499
RP-ID  :  W-7405-ENG-48
RP-ID  :  15007313

美国|英语

来源: UNT Digital Library

【 摘 要 】

We used high resolution Atomic Force Microscopy (AFM) to image compaction of linear and circular DNA by the yeast mitochondrial protein ABF2p , which plays a major role in maintaining mitochondrial DNA. AFM images show that protein binding induces drastic bends in the DNA backbone for both linear and circular DNA. At high concentration of ABF2p DNA collapses into a tight globular structure. We quantified the compaction of linear DNA by measuring the end-to-end distance of the DNA molecule at increasing concentrations of ABF2p. We also derived a polymer statistical mechanics model that gives quantitative description of compaction observed in our experiments. This model shows that a number of sharp bends in the DNA backbone is often sufficient to cause DNA compaction. Comparison of our model with the experimental data showed excellent quantitative correlation and allowed us to determine binding characteristics for ABF2. Our studies indicate that ABF2 compacts DNA through a novel mechanism that involves bending of DNA backbone. We discuss the implications of such a mechanism for mitochondrial DNA maintenance.