Susceptibility of human Plasmodium knowlesi infections to anti-malarials

Malaria Journal

Research

Clemens HM Kocken¹  Erica M Pasini¹  Farrah A Fatih²  Henry M Staines²  Sanjeev Krishna³  Balbir Singh⁴  Mohammed Atique Ahmed⁴  Angela Siner⁴  Janet Cox-Singh⁵  Lu Chan Woon⁶ 

[1] Biomedical Primate Research Centre, Lange Kleiweg 161, GJ Rijswijk, The Netherlands;Division of Clinical Sciences, Centre for Infection and Immunity, St. George’s, University of London, SW17 0RE, London, UK;Division of Clinical Sciences, Centre for Infection and Immunity, St. George’s, University of London, SW17 0RE, London, UK;Malaria Research Centre, University Malaysia Sarawak, 93150, Kuching, Malaysia;Malaria Research Centre, University Malaysia Sarawak, 93150, Kuching, Malaysia;Malaria Research Centre, University Malaysia Sarawak, 93150, Kuching, Malaysia;School of Medicine, University of St Andrews, Medical and Biological Sciences Building, North Haugh, KY16 9TF, St Andrews, UK;Pathology Laboratory, Hospital Sarikei, 96100, Sarikei, Malaysia;

关键词: Artemisinin;    Artemether;    Artesunate;    Dihydroartemisinin;    DHA;    Chloroquine;    Mefloquine;    Malaria;   

DOI  :  10.1186/1475-2875-12-425

 received in 2013-09-18, accepted in 2013-11-12,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundEvidence suggests that Plasmodium knowlesi malaria in Sarawak, Malaysian Borneo remains zoonotic, meaning anti-malarial drug resistance is unlikely to have developed in the absence of drug selection pressure. Therefore, adequate response to available anti-malarial treatments is assumed.MethodsHere the ex vivo sensitivity of human P. knowlesi isolates in Malaysian Borneo were studied, using a WHO schizont maturation assay modified to accommodate the quotidian life cycle of this parasite. The in vitro sensitivities of P. knowlesi H strain adapted from a primate infection to in vitro culture (by measuring the production of Plasmodium lactate dehydrogenase) were also examined together with some assays using Plasmodium falciparum and Plasmodium vivax.ResultsPlasmodium knowlesi is uniformly highly sensitive to artemisinins, variably and moderately sensitive to chloroquine, and less sensitive to mefloquine.ConclusionsTaken together with reports of clinical failures when P. knowlesi is treated with mefloquine, the data suggest that caution is required if using mefloquine in prevention or treatment of P. knowlesi infections, until further studies are undertaken.

【 授权许可】

Unknown   
© Fatih et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.