Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus

BMC Microbiology

Research

Jana Dorazilova¹  Lucy Vojtova¹  Vedran Milosavljevic²  Ludmila Kosaristanova²  Vojtech Adam²  Ludek Zurek²  Kristyna Dolezelikova²  Pavel Svec²  Frantiska Sucha²  Martin Rihacek²  Zdenek Patocka³  Peter Antal⁴  Pavel Kopel⁴ 

[1] Central European Institute of Technology, University of Technology, Brno, Czech Republic;Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic;Department of Forest Management and Applied Geoinformatics, Faculty of Forestry and Wood Technology, Mendel University in Brno, Brno, Czech Republic;Department of Inorganic Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic;

关键词: Staphylococcus aureus;    Iron complex;    Ampicillin;    Synergy;    Antimicrobial activity;   

DOI  :  10.1186/s12866-023-03034-1

 received in 2023-05-08, accepted in 2023-09-28,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectivesResistance to antibiotics among bacteria of clinical importance, including Staphylococcus aureus, is a serious problem worldwide and the search for alternatives is needed. Some metal complexes have antibacterial properties and when combined with antibiotics, they may increase bacterial sensitivity to antimicrobials. In this study, we synthesized the iron complex and tested it in combination with ampicillin (Fe16 + AMP) against S. aureus.MethodsAn iron complex (Fe16) was synthesized and characterized using spectroscopy methods. Confirmation of the synergistic effect between the iron complex (Fe16) and ampicillin (AMP) was performed using ζ–potential, infrared spectra and FICI index calculated from the minimum inhibitory concentration (MIC) from the checkerboard assay. Cytotoxic properties of combination Fe16 + AMP was evaluated on eukaryotic cell line. Impact of combination Fe16 + AMP on chosen genes of S. aureus were performed by Quantitative Real-Time PCR.ResultsThe MIC of Fe16 + AMP was significantly lower than that of AMP and Fe16 alone. Furthermore, the infrared spectroscopy revealed the change in the ζ–potential of Fe16 + AMP. We demonstrated the ability of Fe16 + AMP to disrupt the bacterial membrane of S. aureus and that likely allowed for better absorption of AMP. In addition, the change in gene expression of bacterial efflux pumps at the sub-inhibitory concentration of AMP suggests an insufficient import of iron into the bacterial cell. At the same time, Fe16 + AMP did not have any cytotoxic effects on keratinocytes.ConclusionsCombined Fe16 + AMP therapy demonstrated significant synergistic and antimicrobial effects against S. aureus. This study supports the potential of combination therapy and further research.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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