Cellular and humoral immune responses against the Plasmodium vivax MSP-119 malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil

Malaria Journal

Research

Irene S Soares¹  Maurício Martins Rodrigues²  Vitor Ennes-Vidal³  Evelyn KP Riccio⁴  Paulo RR Totino⁴  Lilian R Pratt-Riccio⁴  Cláudio Tadeu Daniel-Ribeiro⁴  Maria de Fátima Ferreira-da-Cruz⁴  José Maria de Souza⁵ 

[1] Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo, São Paulo, Brazil;Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil;Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil;Laboratório de Pesquisas em Malária, Instituto Oswaldo Cruz, Fiocruz, CEP: 21040-900, Avenida Brasil 4365, ManguinhosRio de Janeiro, RJ, Brazil;Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Reference Center for Malaria in the Extra-Amazonian Region for the Secretary for Health Surveillance from the Ministry of Health, Rio de Janeiro, RJ, Brazil;Programa de Ensaios Clínicos em Malária - Instituto Evandro Chagas, SVS, Belém, Brazil;

关键词: Malaria;    Plasmodium vivax;    MSP-1;    Cellular response;    Antibodies;   

DOI  :  10.1186/1475-2875-12-326

 received in 2013-07-23, accepted in 2013-09-10,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPlasmodium vivax merozoite surface protein-1 (MSP-1) is an antigen considered to be one of the leading malaria vaccine candidates. PvMSP-1 is highly immunogenic and evidences suggest that it is target for protective immunity against asexual blood stages of malaria parasites. Thus, this study aims to evaluate the acquired cellular and antibody immune responses against PvMSP-1 in individuals naturally exposed to malaria infections in a malaria-endemic area in the north-eastern Amazon region of Brazil.MethodsThe study was carried out in Paragominas, Pará State, in the Brazilian Amazon. Blood samples were collected from 35 individuals with uncomplicated malaria. Peripheral blood mononuclear cells were isolated and the cellular proliferation and activation was analysed in presence of 19 kDa fragment of MSP-1 (PvMSP-119) and Plasmodium falciparum PSS1 crude antigen. Antibodies IgE, IgM, IgG and IgG subclass and the levels of TNF, IFN-γ and IL-10 were measured by enzyme-linked immunosorbent assay.ResultsThe prevalence of activated CD4+ was greater than CD8+ T cells, in both ex-vivo and in 96 h culture in presence of PvMSP-119 and PSS1 antigen. A low proliferative response against PvMSP-119 and PSS1 crude antigen after 96 h culture was observed. High plasmatic levels of IFN-γ and IL-10 as well as lower TNF levels were also detected in malaria patients. However, in the 96 h supernatant culture, the dynamics of cytokine responses differed from those depicted on plasma assays; in presence of PvMSP-119 stimulus, higher levels of TNF were noted in supernatant 96 h culture of malaria patient’s cells while low levels of IFN-γ and IL-10 were verified. High frequency of malaria patients presenting antibodies against PvMSP-119 was evidenced, regardless class or IgG subclass.PvMSP-119-induced antibodies were predominantly on non-cytophilic subclasses.ConclusionsThe results presented here shows that PvMSP-119 was able to induce a high cellular activation, leading to production of TNF and emphasizes the high immunogenicity of PvMSP-119 in naturally exposed individuals and, therefore, its potential as a malaria vaccine candidate.

【 授权许可】

Unknown   
© Riccio et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.