Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests

Malaria Journal

Research

Veena Pande¹  Neelima Mishra²  Ridhima Kapoor²  Neena Valecha²  Navin Kumar²  Anupkumar R Anvikar²  Jai PN Singh²  Bina Srivastava² 

[1] Department of Biotechnology, Kumaun University, Nainital, Uttarakhand, India;National Institute of Malaria Research (ICMR), Sector 8, 110077, Dwarka, New Delhi, India;

关键词: Plasmodium falciparum;    Plasmodium falciparum;    Rapid Diagnostic Tests;    Genetic polymorphism;    India;   

DOI  :  10.1186/1475-2875-11-298

 received in 2012-05-28, accepted in 2012-07-31,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundRapid diagnostic tests (RDTs) have revolutionized the diagnosis of malaria. Among the various factors affecting RDTs sensitivity is genetic variation of the antigen used. The genetic variation in PfHRP2 and PfHRP3 proteins was studied among the Indian Plasmodium falciparum isolates.MethodsOne hundred and forty isolates of P. falciparum were collected from six geographical regions of India. Target genes encoding PfHRP2 and PfHRP3 antigens were sequenced to study genetic polymorphism. Minimum detection limit giving a positive rapid diagnostic test was also determined.ResultsExtensive variations were observed in amino acid repeat types of PfHRP2 and PfHRP3. PfHRP2 exhibited more polymorphism than PfHRP3. Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs.ConclusionThe results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.

【 授权许可】

Unknown   
© Kumar et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.