Identification of non-invasive miRNAs biomarkers for prostate cancer by deep sequencing analysis of urinary exosomes

Molecular Cancer

Letter to the Editor

Alicia Llorente¹  Nina P. Hessvik¹  Marta Rodríguez¹  Kirsten Sandvig²  Bastian Fromm³  Susanne Lorenz³  Viktor Berge⁴  Cristina Bajo-Santos⁵  Aija Linē⁵ 

[1] Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital, 0379, Oslo, Norway;Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, 0379, Oslo, Norway;Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital, 0379, Oslo, Norway;Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, 0379, Oslo, Norway;Department of Biosciences, University of Oslo, 0316, Oslo, Norway;Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital, 0379, Oslo, Norway;Department of Urology, Oslo University Hospital, 0586, Oslo, Norway;Latvian Biomedical Research and Study Centre, LV-1067, Riga, Latvia;

关键词: Biomarkers;    Exosomes;    Extracellular vesicles;    microRNAs;    Microvesicles;    noncodingRNA;    Next-generation sequencing;    Prostate cancer;    Small RNA;    Urine;   

DOI  :  10.1186/s12943-017-0726-4

 received in 2017-06-25, accepted in 2017-09-27,  发布年份 2017

来源: Springer

【 摘 要 】

The aim of this study was to identify microRNAs in urinary exosomes that are differently expressed in prostate cancer patients and healthy donors. For this purpose, RNA was extracted from urinary exosomes from 20 prostate cancer patients and 9 healthy males and the microRNAs were analyzed by next generation sequencing. Interestingly, 5 microRNAs – miR-196a-5p, miR-34a-5p, miR-143-3p, miR-501-3p and miR-92a-1-5p – were significantly downregulated in exosomes from prostate cancer patients. Furthermore, RT-qPCR analysis of an independent cohort of 28 prostate cancer patients and 19 healthy males confirmed that miR-196a-5p and miR-501-3p were downregulated in prostate cancer samples. These results suggest that specific microRNAs in urinary exosomes might serve as non-invasive biomarkers for prostate cancer. In particular, miR-196a-5p and miR-501-3p are promising biomarkers that need to be further studied in large patient cohorts.

【 授权许可】

CC BY   
© The Author(s). 2017