Identification of non-invasive miRNAs biomarkers for prostate cancer by deep sequencing analysis of urinary exosomes

Molecular Cancer

Nina P. Hessvik¹  Marta Rodríguez¹  Kirsten Sandvig¹  Alicia Llorente¹  Susanne Lorenz²  Bastian Fromm²  Viktor Berge³  Cristina Bajo-Santos⁴  Aija Linē⁴ 

[1] Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital;Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital;Department of Urology, Oslo University Hospital;Latvian Biomedical Research and Study Centre;

关键词: Biomarkers;    Exosomes;    Extracellular vesicles;    microRNAs;    Microvesicles;    noncodingRNA;   

DOI  :  10.1186/s12943-017-0726-4

来源: DOAJ

【 摘 要 】

Abstract The aim of this study was to identify microRNAs in urinary exosomes that are differently expressed in prostate cancer patients and healthy donors. For this purpose, RNA was extracted from urinary exosomes from 20 prostate cancer patients and 9 healthy males and the microRNAs were analyzed by next generation sequencing. Interestingly, 5 microRNAs – miR-196a-5p, miR-34a-5p, miR-143-3p, miR-501-3p and miR-92a-1-5p – were significantly downregulated in exosomes from prostate cancer patients. Furthermore, RT-qPCR analysis of an independent cohort of 28 prostate cancer patients and 19 healthy males confirmed that miR-196a-5p and miR-501-3p were downregulated in prostate cancer samples. These results suggest that specific microRNAs in urinary exosomes might serve as non-invasive biomarkers for prostate cancer. In particular, miR-196a-5p and miR-501-3p are promising biomarkers that need to be further studied in large patient cohorts.

【 授权许可】

Unknown