Anaerobic bacteria growth in the presence of cathelicidin LL-37 and selected ceragenins delivered as magnetic nanoparticles cargo

BMC Microbiology

Research Article

Paul B. Savage¹  Ewelina Piktel²  Katarzyna Niemirowicz²  Robert Bucki²  Bonita Durnaś³  Stanisław Góźdź⁴  Marzena Wątek⁴  Tomasz Wollny⁵  Jolanta Smok-Kalwat⁵ 

[1] Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA;Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Mickiewicza 2C, Białystok, Poland;Department of Microbiology and Immunology, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, Kielce, Poland;Department of Microbiology and Immunology, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, Kielce, Poland;Holy Cross Oncology Center of Kielce, Artwińskiego 3, Kielce, Poland;Holy Cross Oncology Center of Kielce, Artwińskiego 3, Kielce, Poland;

关键词: Anaerobic bacteria;    Ceragenins;    Cathelicidin;    Magnetic nanoparticles;   

DOI  :  10.1186/s12866-017-1075-6

 received in 2017-03-30, accepted in 2017-07-18,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCationic antibacterial peptides (CAPs) and synthetic molecules mimicking the amphiphilic structure of CAPs, such as ceragenins, are promising compounds for the development of new antimicrobials.ResultsWe tested the in vitro activity of ceragenins CSA-13 and CSA-131 against several anaerobic bacteria including Bacteroides spp. and Clostridium difficile. We compared results to the activity of cathelicidin LL-37, metronidazole and nanosystems developed by attachment of CSA-13 and CSA-131 to magnetic nanoparticles (MNPs). The antibacterial effect was tested using killing assay and modified CLSI broth microdilution assay. Ceragenins CSA-13 and CSA-131 displayed stronger bactericidal activity than LL-37 or metronidazole against all of the tested bacterial strains. Additionally CSA-131 revealed an enhanced ability to prevent the formation of Bacteroides fragilis and Propionibacterium acnes biofilms.ConclusionsThese data confirmed that ceragenins display antimicrobial activity against a broad range of microorganisms including anaerobic bacteria and deserve further investigations as compounds serving to develop new treatment against anaerobic and mixed infections.

【 授权许可】

CC BY   
© The Author(s). 2017