Anaerobic bacteria growth in the presence of cathelicidin LL-37 and selected ceragenins delivered as magnetic nanoparticles cargo

BMC Microbiology

Paul B. Savage¹  Robert Bucki²  Katarzyna Niemirowicz²  Ewelina Piktel²  Stanisław Góźdź³  Marzena Wątek³  Bonita Durnaś³  Jolanta Smok-Kalwat⁴  Tomasz Wollny⁴ 

[1] Department of Chemistry and Biochemistry, Brigham Young University;Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok;Department of Microbiology and Immunology, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce;Holy Cross Oncology Center of Kielce;

关键词: Anaerobic bacteria;    Ceragenins;    Cathelicidin;    Magnetic nanoparticles;   

DOI  :  10.1186/s12866-017-1075-6

来源: DOAJ

【 摘 要 】

Abstract Background Cationic antibacterial peptides (CAPs) and synthetic molecules mimicking the amphiphilic structure of CAPs, such as ceragenins, are promising compounds for the development of new antimicrobials. Results We tested the in vitro activity of ceragenins CSA-13 and CSA-131 against several anaerobic bacteria including Bacteroides spp. and Clostridium difficile. We compared results to the activity of cathelicidin LL-37, metronidazole and nanosystems developed by attachment of CSA-13 and CSA-131 to magnetic nanoparticles (MNPs). The antibacterial effect was tested using killing assay and modified CLSI broth microdilution assay. Ceragenins CSA-13 and CSA-131 displayed stronger bactericidal activity than LL-37 or metronidazole against all of the tested bacterial strains. Additionally CSA-131 revealed an enhanced ability to prevent the formation of Bacteroides fragilis and Propionibacterium acnes biofilms. Conclusions These data confirmed that ceragenins display antimicrobial activity against a broad range of microorganisms including anaerobic bacteria and deserve further investigations as compounds serving to develop new treatment against anaerobic and mixed infections.

【 授权许可】

Unknown