Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways

Natural Products and Bioprospecting

Original Article

Kennedy Kwami Edem Kukuia¹  Benjamin Kingsley Harley²  David Oteng³  Ernest Cudjoe Anorbor³  Augustine Tandoh³  Eric Woode³  Donatus Wewura Adongo³  Charles Kwaku Benneh³  Emmanuel Aduboffour Appiah³  Priscilla Kolibea Mante⁴  Robert Peter Biney⁵ 

[1] Department of Medical Pharmacology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Korle Bu, Accra, Ghana;Department of Pharmacognosy and Herbal Medicine, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana;Department of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana;Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Department of Pharmacotherapeutics and Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana;

关键词: Anxiety disorders;    Pseudospondias microcarpa;    Zebrafish;    Novel tank;    Benzodiazepines;   

DOI  :  10.1007/s13659-023-00399-8

 received in 2023-07-11, accepted in 2023-09-13,  发布年份 2023

来源: Springer

【 摘 要 】

Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases. The hydroethanolic extract (PME) from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models. However, its effects in chronic anxiety models and possible mechanism(s) of action were not studied. Therefore, the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety. The zebrafish light dark test (LDT) and novel tank test (NTT) were employed to assess the anxiolytic-like effects of PME (0.1, 0.3, 1.0 mg mL−1), fluoxetine (3 × 10−5 mg mL−1) and diazepam (1.5 × 10−7 mg mL−1). The chronic unpredictable stress (CUS) test was used to further evaluate the extract’s anxiolytic-like properties. The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil, granisetron, methysergide, or pizotifen, all at 1 × 10−3 mg mL−1. The extract significantly decreased anxiety behaviours in the NT and LD tests. These observed effects of the extract were however counteracted by flumazenil, granisetron, methysergide and pizotifen pre-treatment. In addition, PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish. Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways.Graphical abstract

【 授权许可】

CC BY   
© Kunming Institute of Botany, CAS 2023

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