Transauricular balloon angioplasty in rabbit thoracic aorta: a novel model of experimental restenosis

Lipids in Health and Disease

Research

Dimitrios Alexopoulos¹  Efstratios Apostolakis²  Dimitrios Dougenis³  Ioanna Koniari⁴  Helen Papadaki⁵  Dimitrios Siablis⁶  Athanasios Diamantopoulos⁶  Dimitrios Karnabatidis⁶  Lena Costaridou⁷  Anna Karahaliou⁷  Apostolos Papalois⁸  Evangelia Papadimitriou⁹  Evangelia Poimenidi⁹ 

[1] Cardiology Department of Patras University Hospital, Rion Patras, Greece;Cardiothoracic Surgery Department of Ioannina University Hospital, Ioannina, Greece;Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip, 25500, Greece;Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip, 25500, Greece;Cardiology Department of Patras University Hospital, Rion Patras, Greece;Department of Anatomy, School of Medicine, University of Patras, Patras, Greece;Department of Diagnostic and Interventional Radiology, Patras University Hospital, School of Medicine, Rion Patras, Greece;Department of Medical Physics, School of Medicine, University of Patras, Patras, Greece;Experimental-Research Center ELPEN Pharmaceuticals, Athens, Greece;Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, Patras, Greece;

关键词: Atherosclerosis;    Restenosis;    Hypercholesterolemic diet;    Balloon angioplasty;    Oxidative stress;    Remodelling;    Intima/media ratio;   

DOI  :  10.1186/1476-511X-13-33

 received in 2014-01-02, accepted in 2014-02-05,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to demonstrate a percutaneous transauricular method of balloon angioplasty in high-cholesterol fed rabbits, as an innovative atherosclerosis model.MethodsTwenty male New Zealand rabbits were randomly divided into two groups of ten animals, as follows: atherogenic diet plus balloon angioplasty (group A) and atherogenic diet alone (group B). Balloon angioplasty was performed in the descending thoracic aorta through percutaneous catheterization of the auricular artery. Eight additional animals fed regular diet were served as long term control. At the end of 9 week period, rabbits were euthanized and thoracic aortas were isolated for histological, immunohistochemical and biochemical analysis.ResultsAtherogenic diet induced severe hypercholesterolemia in both group A and B (2802 ± 188.59 and 4423 ± 493.39 mg/dl respectively) compared to the control animals (55.5 ± 11.82 mg/dl; P < 0.001). Group A atherosclerotic lesions appeared to be more advanced histologically (20% type IV and 80% type V) compared to group B lesions (50% type III and 50% type IV). Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm2 versus 12205 ± 8789.23 μm2; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm2; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm2; P = 0.022). Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively). No deaths or systemic complications were reported.ConclusionTransauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

【 授权许可】

CC BY   
© Koniari et al.; licensee BioMed Central Ltd. 2014