Transauricular balloon angioplasty in rabbit thoracic aorta: a novel model of experimental restenosis

Lipids in Health and Disease

Dimitrios Alexopoulos⁵  Dimitrios Dougenis¹  Dimitrios Siablis³  Apostolos Papalois⁴  Lena Costaridou⁶  Anna Karahaliou⁶  Dimitrios Karnabatidis³  Evangelia Poimenidi⁷  Evangelia Papadimitriou⁷  Helen Papadaki²  Athanasios Diamantopoulos³  Efstratios Apostolakis⁸  Ioanna Koniari⁵ 

[1] Cardiothoracic Surgery Department, University Hospital of Patras, Rion Patras zip 25500, Greece;Department of Anatomy, School of Medicine, University of Patras, Patras, Greece;Department of Diagnostic and Interventional Radiology, Patras University Hospital, School of Medicine, Rion Patras, Greece;Experimental-Research Center ELPEN Pharmaceuticals, Athens, Greece;Cardiology Department of Patras University Hospital, Rion Patras, Greece;Department of Medical Physics, School of Medicine, University of Patras, Patras, Greece;Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, Patras, Greece;Cardiothoracic Surgery Department of Ioannina University Hospital, Ioannina, Greece

关键词: Intima/media ratio;    Remodelling;    Oxidative stress;    Balloon angioplasty;    Hypercholesterolemic diet;    Restenosis;    Atherosclerosis;   

Others  :  811369
DOI  :  10.1186/1476-511X-13-33

 received in 2014-01-02, accepted in 2014-02-05,  发布年份 2014

【 摘 要 】

Background

The aim of this study was to demonstrate a percutaneous transauricular method of balloon angioplasty in high-cholesterol fed rabbits, as an innovative atherosclerosis model.

Methods

Twenty male New Zealand rabbits were randomly divided into two groups of ten animals, as follows: atherogenic diet plus balloon angioplasty (group A) and atherogenic diet alone (group B). Balloon angioplasty was performed in the descending thoracic aorta through percutaneous catheterization of the auricular artery. Eight additional animals fed regular diet were served as long term control. At the end of 9 week period, rabbits were euthanized and thoracic aortas were isolated for histological, immunohistochemical and biochemical analysis.

Results

Atherogenic diet induced severe hypercholesterolemia in both group A and B (2802 ± 188.59 and 4423 ± 493.39 mg/dl respectively) compared to the control animals (55.5 ± 11.82 mg/dl; P < 0.001). Group A atherosclerotic lesions appeared to be more advanced histologically (20% type IV and 80% type V) compared to group B lesions (50% type III and 50% type IV). Group A compared to group B atherosclerotic lesions demonstrated similar percentage of macrophages (79.5 ± 9.56% versus 84 ± 12.2%; P = 0.869), more smooth muscle cells (61 ± 14.10% versus 40.5 ± 17.07; P = 0.027), increased intima/media ratio (1.20 ± 0.50 versus 0.62 ± 0.13; P = 0.015) despite the similar degree of intimal hyperplasia (9768 ± 1826.79 μm² versus 12205 ± 8789.23 μm²; P = 0.796), and further significant lumen deterioration (23722 ± 4508.11 versus 41967 ± 20344.61 μm²; P = 0.05) and total vessel area reduction (42350 ± 5819.70 versus 73190 ± 38902.79 μm²; P = 0.022). Group A and B animals revealed similar nitrated protein percentage (P = NS), but significantly higher protein nitration compared to control group (P < 0.01; P < 0.01, respectively). No deaths or systemic complications were reported.

Conclusion

Transauricular balloon angioplasty constitutes a safe, minimally invasive and highly successful model of induced atherosclerosis in hyperlipidaemic rabbits.

【 授权许可】

   
2014 Koniari et al.; licensee BioMed Central Ltd.

【 图 表 】

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