Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo

Malaria Journal

Research

Michel Bitemo¹  Pembe Issamou Mayengue²  Freddy Vladimir Malonga²  Francine Ntoumi³  Mathieu Ndounga⁴ 

[1] Centre de Recherche et d'Etude en Sciences Sociales et Humaines, BP 2019, Brazzaville, Republic of Congo;Fondation Congolaise pour la Recherche Médicale, BP 2672, Brazzaville, Republic of Congo;Faculty of Health Sciences, University Marien Ngouabi, BP 2672, Brazzaville, Republic of Congo;Fondation Congolaise pour la Recherche Médicale, BP 2672, Brazzaville, Republic of Congo;Faculty of Health Sciences, University Marien Ngouabi, BP 2672, Brazzaville, Republic of Congo;Institute for Tropical Medicine, University of Tübingen, 72074, Tübingen, Germany;Laboratoire de Pharmacologie, Centre d'Etudes sur les Ressources Végétales, BP 1249, Brazzaville, Republic of Congo;

关键词: Malaria;    Parasite Density;    Uncomplicated Malaria;    Malaria Control Intervention;    Parasite Genotype;   

DOI  :  10.1186/1475-2875-10-276

 received in 2011-06-10, accepted in 2011-09-22,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe characterization of malaria parasite populations circulating in an area is part of site characterization, as a basis for evaluating the impact of malaria interventions on genetic diversity, parasite species, and multiplicity of infection. The present study was aimed at analysing genetic diversity of Plasmodium falciparum merozoite surface proteins 1 and 2 (MSP-1 and MSP-2) and to determine the multiplicity of infection in clinical isolates collected from children living in the Southern district of Brazzaville in the Republic of Congo.MethodsA total of 125 isolates from patients with uncomplicated malaria attending Terinkyo and Madibou health centres were collected between January and June 2005 while evaluating the therapeutic efficacy of amodiaquine-artesunate combination. DNA was extracted and msp-1 and msp-2 genes were genotyped using allele-specific nested-PCR.ResultsOut of 468 distinct fragments detected, 15 msp-1 and 20 msp-2 genotypes were identified. For the msp-1 gene, K1 family was the predominant allelic type carried alone or in association with RO33 and Mad20 types, whereas the 3D7 family was the most prevalent in the msp-2 gene. Overall, the mean multiplicity of infection was 2.2. Out of 125 samples, 104 (83%) harboured more than one parasite genotype. There was no statistical significant difference in the multiplicity of infection by either sex or age of patients. However, a statistically significant correlation was found between parasite densities and the number of genotypes.ConclusionPolymorphism in P. falciparum clinical isolates from Brazzaville was high and mainly of multiple clones. The basis for the positive association between parasite densities and multiplicity of infection is discussed.

【 授权许可】

Unknown   
© Mayengue et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.