Production of d-lactate from glucose using Klebsiella pneumoniae mutants

Microbial Cell Factories

Research

Xiaojuan Han¹  Huizhou Liu¹  Guang Zhao¹  Mo Xian¹  Xinjun Feng²  Xiutao Liu³  Zhiqiang Zhao³  Liqun Jiang⁴ 

[1] CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China;CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China;CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, 510640, Guangzhou, China;CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China;University of Chinese Academy of Sciences, 100049, Beijing, China;CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, 510640, Guangzhou, China;

关键词: -Lactate;    Acetolactate synthase;    Acetate kinase;    Alcohol dehydrogenase;    Klebsiella pneumoniae;   

DOI  :  10.1186/s12934-017-0822-6

 received in 2017-09-16, accepted in 2017-11-12,  发布年份 2017

来源: Springer

【 摘 要 】

Backgroundd-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for d-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of d-lactate.ResultsBased on the redirection of carbon towards by-product formation, the effects of single-gene and multiple-gene deletions in K. pneumoniae on d-lactate production from glucose via acetolactate synthase (budB), acetate kinase (ackA), and alcohol dehydrogenase (adhE) were tested. Klebsiella pneumoniae mutants had different production behaviours. The accumulation of the main by-products was decreased in the mutants. The triple mutant strain had the most powerful ability to produce optically pure d-lactate from glucose, and was tested with xylose and arabinose as carbon sources. Fed-batch fermentation was also carried out under various aeration rates, and the strain accumulated 125.1 g/L d-lactate with a yield of 0.91 g/g glucose at 2.5 vvm.ConclusionsKnocking out by-product synthesis genes had a remarkable influence on the production and yield of d-lactate. This study demonstrated, for the first time, that K. pneumoniae has great potential to convert monosaccharides into d-lactate. The results provide new insights for industrial production of d-lactate by K. pneumoniae.

【 授权许可】

CC BY   
© The Author(s) 2017