Natural variation in virulence of the entomopathogenic fungus Beauveria bassiana against malaria mosquitoes

Malaria Journal

Research

Willem Takken¹  Constantianus JM Koenraadt¹  Alfons JM Debets²  Sijmen E Schoustra²  Bas J Zwaan²  Claudio A Valero-Jiménez³  Jan AL van Kan⁴ 

[1] Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700Wageningen, EH, The Netherlands;Laboratory of Genetics, Wageningen University, P.O Box 309, 6700, Wageningen, AH, The Netherlands;Laboratory of Genetics, Wageningen University, P.O Box 309, 6700, Wageningen, AH, The Netherlands;Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700Wageningen, EH, The Netherlands;Laboratory of Phytopathology, Wageningen University, P.O Box 8025, 6700Wageningen, EE, The Netherlands;

关键词: Entomopathogenic fungi;    Beauveria bassiana;    Vector control;    Virulence;   

DOI  :  10.1186/1475-2875-13-479

 received in 2014-07-09, accepted in 2014-11-30,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundInsecticide resistance is greatly hampering current efforts to control malaria and therefore alternative methods are needed. Entomopathogenic fungi have been proposed as an alternative with a special focus on the cosmopolitan species Beauveria bassiana. However, few studies have analysed the effects of natural variation within fungal isolates on mosquito survival, and the implications and possible exploitation for malaria control.MethodsLaboratory bioassays were performed on adult female mosquitoes (Anopheles coluzzii) with spores from 29 isolates of B. bassiana, originating from different parts of the world. In addition, phenotypic characteristics of the fungal isolates such as sporulation, spore size and growth rate were studied to explore their relationship with virulence.ResultsAll tested isolates of B. bassiana killed An. coluzzii mosquitoes, and the rate at which this happened differed significantly among the isolates. The risk of mosquitoes dying was around ten times higher when they were exposed to the most virulent as compared to the least virulent isolate. There was significant variation among isolates in spore size, growth rate and sporulation, but none of these morphological characteristics were correlated, and thus predictive, for the ability of the fungal isolate to kill malaria mosquitoes.ConclusionsThis study shows that there is a wide natural variation in virulence of isolates of B. bassiana, and that selecting an appropriate fungal isolate is highly relevant in killing and thus controlling malaria mosquitoes, particularly if used as part of an integrated vector management strategy. Also, the wide variation observed in virulence offers the opportunity to better understand the molecular and genetic mechanisms that drive this variation and thus to address the potential development of resistance against entomopathogenic fungi.

【 授权许可】

CC BY   
© Valero-Jiménez et al.; licensee BioMed Central. 2014