Laboratory Evaluation of The Selected Entomopathogenic Fungi and Bacteria Against Larval and Pupal Stages of Spodoptera litura L

Humanosphere Science School 2017; 7th International Symposium for a Sustainable Humanosphere

地球科学;经济学

Zulfitri, A.^1 ; Lestari, A.S.^1 ; Krishanti, N.P.R.A.^1 ; Zulfiana, D.^1

Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, Cibinong

16911, Indonesia^1

关键词: Beauveria bassiana;    Cell Suspension;    Dipping method;    Entomopathogenic fungus;    Laboratory evaluation;    Larval stage;    Pre-mixing method;    Spodoptera litura;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/166/1/012009/pdf
DOI  :  10.1088/1755-1315/166/1/012009

来源: IOP

【 摘 要 】

In the present study, efficacy of selected entomopathogenic fungi and bacteria against Spodoptera litura were evaluated. The study used two life phase of the insect, third to fourth larval and pupal stages. Evaluation on larval stage used larvae-dipping method while soil premixing method was used in efficacy test against S. litura pupae. Spore solution of five entomopathogenic fungals (Paecilomyces sp, Beauveria bassiana, T2B, B2 and T4B), cell suspension of 1 entomopathogenic bacteria (Serratia sp.) and suspension solution of consortium between T2B and Serratia sp. were used. Evaluation on larval stage showed the highest mortality was found in B2 treatment (70%), followed by Serratia sp. (60%) and consortium (50%) treatment. While on S. litura pupal stage evaluation, consortium treatment resulted in 62,5% of tested pupae failed to become adult moth. Other treatments on pupal stage evaluation showed under 40% failure transformation to adult stage.