Isolation, identification and virulence of indigenous entomopathogenic fungal strains against the peach-potato aphid, Myzus persicae Sulzer (Hemiptera: Aphididae), and the fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Egyptian Journal of Biological Pest Control

Nevien M. Gaber¹  Mohamed Alkafafy²  Sami Ullah³  Abu Bakar Muhammad Raza³  Muhammad Zeeshan Majeed³  Muhammad Asam Riaz³  Muhammad Asim⁴  Muhammad Imran Hamid⁴  Samy Sayed⁵ 

[1] Agricultural Research Center, Plant Protection Research Institute, 12311, Dokki, Giza, Egypt;Department of Biotechnology, College of Science, Taif University, B.O. Box 11099, 21944, Taif, Saudi Arabia;Department of Entomology, College of Agriculture, University of Sargodha, 40100, Sargodha, Pakistan;Department of Plant Pathology, College of Agriculture, University of Sargodha, 40100, Sargodha, Pakistan;Department of Science and Technology, University College-Ranyah, Taif University, B.O. Box 11099, 21944, Taif, Saudi Arabia;

关键词: Entomopathogenic fungi;    Indigenous isolates;    Beauveria bassiana;    Metarhizium anisopliae;    Spodoptera frugiperda;    Myzus persicae;   

DOI  :  10.1186/s41938-021-00500-8

来源: Springer

【 摘 要 】

BackgroundAs different biogeographic strains and isolates of entomopathogenic fungi vary in their genetic, enzymatic and pathogenic characteristics, this study assessed the virulence of 2 indigenous strains of Beauveria bassiana (Balsam) Vuillemin and Metarhizium anisopliae (Metschn.) Sorokin (Ascomycota, Hypocreales: Clavicipitaceae), isolated from naturally infected insect cadavers, against the 3rd instar nymphs of Myzus persicae (Sulzer) (Hemiptera: Aphididae) and 3rd instar larvae of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) using leaf-dip and larval-dip methods, respectively.ResultsBoth fungal isolates exhibited considerable pathogenicity against M. persicae and S. frugiperda. Mortality in all bioassays was conidial concentration and exposure time dependent and increased significantly along with both factors (R2 = 0.86–0.99 for B. bassiana and 0.82–0.94 for M. anisopliae). Moreover, M. anisopliae isolate appeared more virulent to S. frugiperda larvae than B. bassiana isolate, while the later fungal isolate was more lethal to M. persicae nymphs than the former one. At the highest conidial concentration (1.0 × 109 conidia/ml), M. anisopliae caused maximum mean mortality of S. frugiperda (88%) and M. persicae (65%) and B. bassiana exhibited maximum mean mortality of S. frugiperda (76%) and M. persicae (94%). Moreover, probit regression analyses showed LT50 values for M. persicae of 4.57 and 6.86 days at 1.0 × 109 conidia/ml for the isolates of B. bassiana and M. anisopliae, respectively, while LC50 values were 7.75 × 106 and 8.70 × 107 conidia/ml after 10th day of application, for the isolates of B. bassiana and M. anisopliae, respectively, against M. persicae. Similarly, LT50 values for S. frugiperda were 7.75 and 7.03 days for 1.0 × 109 conidia/ml concentration and LC50 values were 2.84 × 107 and 8.84 × 105 conidia/ml at 10th day data for the isolates of B. bassiana and M. anisopliae, respectively.ConclusionOverall study results demonstrated the effectiveness of B. bassiana and M. anisopliae against M. persicae and S. frugiperda, respectively. However, field evaluations of these indigenously isolated promising fungal strains against these insect pests.

【 授权许可】

CC BY