Conjugated linoleic acid improves oocyte cryosurvival through modulation of the cryoprotectants influx rate

Reproductive Biology and Endocrinology

Research

Maria C. Baptista¹  Carla C. Marques¹  Antonio E. M. Horta¹  Rosa M. L. N. Pereira²  Joana E. Matos³  Graça Soveral⁴  Teresa F. Moura⁵ 

[1] INIAV, Unidade de Biotecnologias e Recursos Genéticos, Quinta da Fonte Boa, 2005-048, Vale de Santarém, Portugal;INIAV, Unidade de Biotecnologias e Recursos Genéticos, Quinta da Fonte Boa, 2005-048, Vale de Santarém, Portugal;Escola Universitária Vasco da Gama, Campus Universitário de Lordemão, 3020-210, Coimbra, Portugal;CIISA, Faculdade de Medicina Veterinária da Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477, Lisbon, Portugal;Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, Universidade de Lisboa, 1649-003, Lisbon, Portugal;Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, Universidade de Lisboa, 1649-003, Lisbon, Portugal;Departamento Bioquímica e Biologia Humana, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal;Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, Universidade de Lisboa, 1649-003, Lisbon, Portugal;Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Monte de Caparica, 2829-516, Caparica, Portugal;

关键词: CLA conjugated linoleic acid;    Oocytes;    Membrane permeability;    Cryopreservation;   

DOI  :  10.1186/s12958-015-0059-3

 received in 2015-02-20, accepted in 2015-06-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIn cryopreservation, oocytes are subjected to extreme hyperosmotic conditions, inducing large volume changes that, along with an abrupt temperature drop, interfere with their developmental competence. Our objectives in this work were to find conditions enabling an increase in oocyte cryosurvival and subsequent development.MethodsAbattoir-derived bovine oocytes were cultured without (Control group) or with trans-10,cis-12 conjugated linoleic acid isomer (CLA group). Comparative observations were made for 1) the oocyte developmental competence after exposure to cryoprotectants followed or not by vitrification/warming, 2) the oocyte membrane permeability to water (using the non-permeant cryoprotectant sucrose) and 3) the oocyte membrane permeability to two cryoprotectants (ethylene glycol, EG, and dimethyl sulfoxide, DMSO). Mature oocytes cultured with or without CLA and vitrified/warmed or only exposed to cryoprotectants without vitrification were subjected to in vitro fertilization; embryo culture proceeded until the blastocyst stage. The oocyte membrane permeabilities to water and cryoprotectants were estimated using mature oocytes subjected to hyperosmotic challenges. For water permeability, 200 mM sucrose was used, whereas for the cryoprotectant permeability, a 10 % solution of both EG and DMSO was used. The data were analyzed using the MIXED procedure and Student’s T-test.ResultsCLA supplementation improves the developmental competence of vitrified/warmed and cryoprotectants exposed oocytes (p < 0.01) and reduces their membrane permeability to water (37 %, p < 0.001) and to cryoprotectants (42 %, p < 0.001).Conclusions: By slowing the fluxes of water and of permeant cryoprotectants, CLA contributed to improved oocyte cryosurvival and post-thawed viability. This isomer supplementation to the maturation media should be considered when designing new protocols for oocyte cryopreservation.

【 授权许可】

Unknown   
© Matos et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.