Metabolism of aromatics by Trichosporon oleaginosus while remaining oleaginous

Microbial Cell Factories

Research

Allison Yaguchi¹  Alana Robinson¹  Mark Blenner¹  Erin Mihealsick¹ 

[1] Department of Chemical and Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., 29634, Clemson, SC, USA;

关键词: Trichosporon oleaginosus;    Aromatics;    Phenol;    Resorcinol;    p-Hydroxybenzoic acid;    Lignin;    Yeast;    Aromatic metabolism;   

DOI  :  10.1186/s12934-017-0820-8

 received in 2017-08-21, accepted in 2017-11-11,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe oleaginous yeast, Trichosporon oleaginosus, has been extensively studied for its ability to metabolize non-conventional feedstocks. These include phenol-containing waste streams, such as distillery wastewater, or streams consisting of non-conventional sugars, such as hydrolyzed biomass and various bagasse. An initial BLAST search suggests this yeast has putative aromatic metabolizing genes. Given the desirability to valorize underutilized feedstocks such as lignin, we investigated the ability of T. oleaginosus to tolerate and metabolize lignin-derived aromatic compounds.ResultsTrichosporon oleaginosus can tolerate and metabolize model lignin monoaromatics and associated intermediates within funneling pathways. Growth rates and biomass yield were similar to glucose when grown in 4-hydroxybenzoic acid (pHBA) and resorcinol, but had an increased lag phase when grown in phenol. Oleaginous behavior was observed using resorcinol as a sole carbon source. Fed-batch feeding resulted in lipid accumulation of 69.5% on a dry weight basis.ConclusionsThough the exact pathway of aromatic metabolism remains to be determined for T. oleaginosus, the results presented in this work motivate use of this organism for lignin valorization and phenolic wastewater bioremediation. Trichosporon oleaginosus is the first yeast shown to be oleaginous while growing on aromatic substrates, and shows great promise as a model industrial microbe for biochemical and biofuel production from depolymerized lignin.

【 授权许可】

CC BY   
© The Author(s) 2017