Enchanced levels of apolipoprotein M during HBV infection feedback suppresses HBV replication

Lipids in Health and Disease

Research

Jin-Gang Gu¹  Yan Xie¹  Xin Zhou¹  Duo-zhi Cheng²  Cheng-liang Zhu³  Fang Liu⁴ 

[1] Center for Gene Diagnosis, Zhongnan Hospital, Wuhan University, 430071, Wuhan, P.R. China;Department of Clinical Laboratory of Taihe Hospital Affiliated to Hubei Medical College, 442000, Shiyan, P.R. China;Department of Clinical Laboratory, Renmin Hospital of Wuhan University, 430060, Wuhan, Wuhan, P.R. China;The State Key Laboratory of Virology, College of Life Sciences, Wuhan University, 430072, Wuhan, P.R. China;

关键词: Hepatitis B virus;    Apolipoprotein M;    ELISA;    RT-PCR;    Replication;   

DOI  :  10.1186/1476-511X-10-154

 received in 2010-10-31, accepted in 2011-08-29,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundChronic liver diseases can interfere with hepatic metabolism of lipoproteins, apolipoproteins. Hepatitis B virus (HBV) is a major etiological agent causing acute and chronic liver diseases. Apolipoprotein M (ApoM) is a high-density lipoprotein (HDL) apolipoprotein and exclusively expressed in the liver parenchyma cells and in the tubular cells of the kidney. This study was to determine the correlation between HBV infection and ApoM expression.Materials and methodsSerum ApoM levels in patients with HBV infection and in healthy individuals were measured by ELISA, ApoM mRNA expression were determined by RT-PCR, and the expression of S and E proteins of HBV, as well as the synthesis of viral DNA were measured by ELISA and real-time PCR.ResultsThe levels of serum ApoM was significantly elevated in patients as compared to healthy individuals (P < 0.001), ApoM promoter activity, mRNA and protein expression were all stimulated in cells transfected with infectious HBV clone. In addition, ApoM decreases the expression of S and E proteins of HBV and the synthesis of viral DNA.ConclusionRaised ApoM levels in HBV infection may in turn suppress HBV replication, one of the protective mechanisms of nature.

【 授权许可】

Unknown   
© Gu et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.