Identification of Salmonella enterica serovar Kentucky genes involved in attachment to chicken skin

BMC Microbiology

Research Article

Attila Karsi¹  Mark L. Lawrence¹  R. Hartford Bailey²  Sanaz Salehi²  Kevin Howe²  John Brooks³ 

[1] Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA;Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA;USDA-ARS, Genetics and Precision Agriculture Unit, Mississippi State, Mississippi, USA;

关键词: Salmonella;    Kentucky;    Flagella;    Poultry;    Skin;    Attachment;   

DOI  :  10.1186/s12866-016-0781-9

 received in 2016-01-22, accepted in 2016-07-15,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundRegardless of sanitation practices implemented to reduce Salmonella prevalence in poultry processing plants, the problem continues to be an issue. To gain an understanding of the attachment mechanism of Salmonella to broiler skin, a bioluminescent-based mutant screening assay was used. A random mutant library of a field-isolated bioluminescent strain of Salmonella enterica serovar Kentucky was constructed. Mutants’ attachment to chicken skin was assessed in 96-well plates containing uniform 6 mm diameter pieces of circular chicken skin. After washing steps, mutants with reduced attachment were selected based on reduced bioluminescence, and transposon insertion sites were identified.ResultsAttachment attenuation was detected in transposon mutants with insertion in genes encoding flagella biosynthesis, lipopolysaccharide core biosynthesis protein, tryptophan biosynthesis, amino acid catabolism pathway, shikimate pathway, tricarboxylic acid (TCA) cycle, conjugative transfer system, multidrug resistant protein, and ATP-binding cassette (ABC) transporter system. In particular, mutations in S. Kentucky flagellar biosynthesis genes (flgA, flgC, flgK, flhB, and flgJ) led to the poorest attachment of the bacterium to skin.ConclusionsThe current study indicates that attachment of Salmonella to broiler skin is a multifactorial process, in which flagella play an important role.

【 授权许可】

CC BY   
© The Author(s). 2016