Bacillus pumilus KatX2 confers enhanced hydrogen peroxide resistance to a Bacillus subtilis PkatA::katX2 mutant strain

Microbial Cell Factories

Research

Andreas Otto¹  Dirk Albrecht¹  Daniela Zühlke¹  Stefan Handtke²  Dörte Becher²  Michael Hecker²  Kathrin Riedel²  Birgit Voigt³  Thomas Schweder⁴ 

[1] Institute for Microbiology, University of Greifswald, 17489, Greifswald, Germany;Institute for Microbiology, University of Greifswald, 17489, Greifswald, Germany;Institute of Marine Biotechnology, 17489, Greifswald, Germany;Institute for Microbiology, University of Greifswald, 17489, Greifswald, Germany;Institute of Marine Biotechnology, 17489, Greifswald, Germany;Research Institute for Leather and Plastic Sheeting, Meißner-Ring 1-5, 09599, Freiberg, Germany;Institute of Pharmacy, University of Greifswald, 17489, Greifswald, Germany;Institute of Marine Biotechnology, 17489, Greifswald, Germany;

关键词: Bacillus pumilus;    Hydrogen peroxide;    Oxidative stress;    Catalase;   

DOI  :  10.1186/s12934-017-0684-y

 received in 2016-12-22, accepted in 2017-04-19,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBacillus pumilus cells exhibit a significantly higher resistance to hydrogen peroxide compared to closely related Bacilli like Bacillus subtilis.ResultsIn this study we analyzed features of the catalase KatX2 of B. pumilus as one of the most important parts of the cellular response to hydrogen peroxide. KatX2, the vegetative catalase expressed in B. pumilus, was compared to the vegetative catalase KatA of B. subtilis. Data of our study demonstrate that B. pumilus can degrade toxic concentrations of hydrogen peroxide faster than B. subtilis. By replacing B. subtiliskatA gene by katX2 we could significantly enhance its resistance to H2O2 and its potential to eliminate this toxic compound. Mutant cells showed a 1.5- to 2-fold higher survival to toxic concentrations of hydrogen peroxide compared to wild type cells. Furthermore, we found reversible but also irreversible oxidations of the KatX2 protein which, in contrast to KatA, contains several cysteine residues.ConclusionsOur study indicates that the catalase KatX2 plays a major role in the increased resistance of B. pumilus to oxidative stress caused by hydrogen peroxide. Resistance to hydrogen peroxide of other Bacilli can be enhanced by exchanging the native catalase in the cells with katX2.

【 授权许可】

CC BY   
© The Author(s) 2017

【 图 表 】

Fig. 6