Palmitoleic acid (n-7) increases white adipocytes GLUT4 content and glucose uptake in association with AMPK activation

Lipids in Health and Disease

Research

Maysa M Cruz¹  Maria Isabel C Alonso-Vale¹  Fabio B Lima²  Andressa Bolsoni-Lopes²  Patricia Chimin²  Talita SM Farias²  Francisco L Torres-Leal²  William T Festuccia²  Paula B Andrade³  Sandro M Hirabara³ 

[1] Department of Biological Sciences, Institute of Environmental Sciences, Chemical and Pharmaceutical, Federal University of Sao Paulo, 210, Sao Nicolau St., 09913-030, Diadema, Brazil;Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil;Institute of Physical Activity Sciences and Sports, Program of Post-Graduate in Human Movement Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil;

关键词: GLUT4;    AMPK;    Akt;    de novo;    Glucose oxidation;    Glucose metabolism;   

DOI  :  10.1186/1476-511X-13-199

 received in 2014-09-10, accepted in 2014-12-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPalmitoleic acid was previously shown to improve glucose homeostasis by reducing hepatic glucose production and by enhancing insulin-stimulated glucose uptake in skeletal muscle. Herein we tested the hypothesis that palmitoleic acid positively modulates glucose uptake and metabolism in adipocytes.MethodsFor this, both differentiated 3 T3-L1 cells treated with either palmitoleic acid (16:1n7, 200 μM) or palmitic acid (16:0, 200 μM) for 24 h and primary adipocytes from mice treated with 16:1n7 (300 mg/kg/day) or oleic acid (18:1n9, 300 mg/kg/day) by gavage for 10 days were evaluated for glucose uptake, oxidation, conversion to lactate and incorporation into fatty acids and glycerol components of TAG along with the activity and expression of lipogenic enzymes.ResultsTreatment of adipocytes with palmitoleic, but not oleic (in vivo) or palmitic (in vitro) acids, increased basal and insulin-stimulated glucose uptake and GLUT4 mRNA levels and protein content. Along with uptake, palmitoleic acid enhanced glucose oxidation (aerobic glycolysis), conversion to lactate (anaerobic glycolysis) and incorporation into glycerol-TAG, but reduced de novo fatty acid synthesis from glucose and acetate and the activity of lipogenic enzymes glucose 6-phosphate dehydrogenase and ATP-citrate lyase. Importantly, palmitoleic acid induction of adipocyte glucose uptake and metabolism were associated with AMPK activation as evidenced by the increased protein content of phospho(p)Thr172AMPKα, but no changes in pSer473Akt and pThr308Akt. Importantly, such increase in GLUT4 content induced by 16:1n7, was prevented by pharmacological inhibition of AMPK with compound C.ConclusionsIn conclusion, palmitoleic acid increases glucose uptake and the GLUT4 content in association with AMPK activation.

【 授权许可】

Unknown   
© Bolsoni-Lopes et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.