Palmitoleic acid (n-7) increases white adipocytes GLUT4 content and glucose uptake in association with AMPK activation

Lipids in Health and Disease

Maria Isabel C Alonso-Vale³  Fabio B Lima²  Sandro M Hirabara¹  Paula B Andrade¹  Maysa M Cruz³  Francisco L Torres-Leal²  Talita SM Farias²  Patricia Chimin²  William T Festuccia²  Andressa Bolsoni-Lopes² 

[1] Institute of Physical Activity Sciences and Sports, Program of Post-Graduate in Human Movement Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil;Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil;Department of Biological Sciences, Institute of Environmental Sciences, Chemical and Pharmaceutical, Federal University of Sao Paulo, 210, Sao Nicolau St., Diadema 09913-030, Brazil

关键词: Glucose metabolism;    Glucose oxidation;    de novo lipogenesis;    Akt;    AMPK;    GLUT4;   

Others  :  1135968
DOI  :  10.1186/1476-511X-13-199

 received in 2014-09-10, accepted in 2014-12-11,  发布年份 2014

【 摘 要 】

Background

Palmitoleic acid was previously shown to improve glucose homeostasis by reducing hepatic glucose production and by enhancing insulin-stimulated glucose uptake in skeletal muscle. Herein we tested the hypothesis that palmitoleic acid positively modulates glucose uptake and metabolism in adipocytes.

Methods

For this, both differentiated 3 T3-L1 cells treated with either palmitoleic acid (16:1n7, 200 μM) or palmitic acid (16:0, 200 μM) for 24 h and primary adipocytes from mice treated with 16:1n7 (300 mg/kg/day) or oleic acid (18:1n9, 300 mg/kg/day) by gavage for 10 days were evaluated for glucose uptake, oxidation, conversion to lactate and incorporation into fatty acids and glycerol components of TAG along with the activity and expression of lipogenic enzymes.

Results

Treatment of adipocytes with palmitoleic, but not oleic (in vivo) or palmitic (in vitro) acids, increased basal and insulin-stimulated glucose uptake and GLUT4 mRNA levels and protein content. Along with uptake, palmitoleic acid enhanced glucose oxidation (aerobic glycolysis), conversion to lactate (anaerobic glycolysis) and incorporation into glycerol-TAG, but reduced de novo fatty acid synthesis from glucose and acetate and the activity of lipogenic enzymes glucose 6-phosphate dehydrogenase and ATP-citrate lyase. Importantly, palmitoleic acid induction of adipocyte glucose uptake and metabolism were associated with AMPK activation as evidenced by the increased protein content of phospho(p)Thr172AMPKα, but no changes in pSer473Akt and pThr308Akt. Importantly, such increase in GLUT4 content induced by 16:1n7, was prevented by pharmacological inhibition of AMPK with compound C.

Conclusions

In conclusion, palmitoleic acid increases glucose uptake and the GLUT4 content in association with AMPK activation.

【 授权许可】

   
2014 Bolsoni-Lopes et al.; licensee BioMed Central.

【 图 表 】

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