Bioluminescent magnetic nanoparticles as potential imaging agents for mammalian spermatozoa

Journal of Nanobiotechnology

Research

Keisha B. Walters¹  Erick S. Vasquez²  Jean M. Feugang³  Scott T. Willard⁴  Peter L. Ryan⁵ 

[1] Dave C. Swalm School of Chemical Engineering, Mississippi State University, 39762, Mississippi State, MS, USA;Department of Chemical and Materials Engineering, University of Dayton, 45469, Dayton, OH, USA;Facility for Cellular and Organismal Imaging, Mississippi State University, 39762, Mississippi State, MS, USA;Department of Animal and Dairy Sciences, Mississippi State University, 39762, Mississippi State, MS, USA;Facility for Cellular and Organismal Imaging, Mississippi State University, 39762, Mississippi State, MS, USA;Department of Animal and Dairy Sciences, Mississippi State University, 39762, Mississippi State, MS, USA;Department of Biochemistry and Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 39762, Mississippi State, MS, USA;Facility for Cellular and Organismal Imaging, Mississippi State University, 39762, Mississippi State, MS, USA;Department of Animal and Dairy Sciences, Mississippi State University, 39762, Mississippi State, MS, USA;Department of Pathology and Population Medicine, College of Veterinary and Medicine, Mississippi State University, 39762, Mississippi State, MS, USA;

关键词: Spermatozoa;    Bioluminescence Imaging;    Magnetic nanoparticles;    Nanocomposites;    Reproduction;    Core–shell nanoparticles;    Luciferase;   

DOI  :  10.1186/s12951-016-0168-y

 received in 2015-11-09, accepted in 2016-02-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundNanoparticles have emerged as key materials for developing applications in nanomedicine, nanobiotechnology, bioimaging and theranostics. Existing bioimaging technologies include bioluminescent resonance energy transfer-conjugated quantum dots (BRET-QDs). Despite the current use of BRET-QDs for bioimaging, there are strong concerns about QD nanocomposites containing cadmium which exhibits potential cellular toxicity.ResultsIn this study, bioluminescent composites comprised of magnetic nanoparticles and firefly luciferase (Photinus pyralis) are examined as potential light-emitting agents for imaging, detection, and tracking mammalian spermatozoa. Characterization was carried out using infrared spectroscopy, TEM and cryo-TEM imaging, and ζ-potential measurements to demonstrate the successful preparation of these nanocomposites. Binding interactions between the synthesized nanoparticles and spermatozoon were characterized using confocal and atomic/magnetic force microscopy. Bioluminescence imaging and UV–visible-NIR microscopy results showed light emission from sperm samples incubated with the firefly luciferase-modified nanoparticles. Therefore, these newly synthesized luciferase-modified magnetic nanoparticles show promise as substitutes for QD labeling, and can potentially also be used for in vivo manipulation and tracking, as well as MRI techniques.ConclusionsThese preliminary data indicate that luciferase-magnetic nanoparticle composites can potentially be used for spermatozoa detection and imaging. Their magnetic properties add additional functionality to allow for manipulation, sorting, or tracking of cells using magnetic techniques.

【 授权许可】

CC BY   
© Vasquez et al. 2016