Collagen-based silver nanoparticles for biological applications: synthesis and characterization

Journal of Nanobiotechnology

Research

Selma AS Kuckelhaus¹  Fernando Lucas Primo²  Antonio C Tedesco²  Graciely Gomides Gobo²  José Ribeiro dos Santos Júnior³  Yvonne P Mascarenhas⁴  Ana C Mafud⁴  Carla Eiras⁵  José Raimundo Corrêa⁶  Adriany Amorin⁷  José Roberto SA Leite⁷  Durcilene Silva⁷  Patrick V Quelemes⁷  Vinicius S Cardoso⁸ 

[1] Area of Morphology, Faculty of Medicine, University of Brasília, 70910900, Brasília, DF, Brazil;Departamento de Química, Laboratório de Fotobiologia e Fotomedicina, Faculdade de Filosofia, Ciências e Letras de Ribeirāo Preto, Universidade de Sāo Paulo, 14040-901, Ribeirāo Preto, SP, Brazil;Department of Chemistry, Campus Teresina, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil;Institute of Physics of Sāo Carlos (IFSC), University of Sāo Paulo (USP), 13566-590, Sāo Carlos, SP, Brazil;Interdisciplinary Laboratory for Advanced Materials (LIMAV), Federal University of Piauí, 64049-550, Teresina, PI, Brazil;Laboratory of Microscopy, Institute of Biology, University of Brasília, 70910900, Brasília, DF, Brazil;Research Center in Biodiversity and Biotechnology (Biotec), Campus Parnaíba, Federal University of Piauí, Av Sāo Sebastian 2819, 64202-020, Parnaíba, Piauí, Brazil;Research Center in Biodiversity and Biotechnology (Biotec), Campus Parnaíba, Federal University of Piauí, Av Sāo Sebastian 2819, 64202-020, Parnaíba, Piauí, Brazil;Physiotherapy Department, Campus Parnaíba, Federal University of Piauí, Av. Sāo Sebastiāo 2819, 64202-020, Parnaíba, Piauí, Brazil;

关键词: Silver nanoparticles;    Collagen;    Antimicrobial activity;    Cell viability;   

DOI  :  10.1186/s12951-014-0036-6

 received in 2014-07-01, accepted in 2014-09-04,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundType I collagen is an abundant natural polymer with several applications in medicine as matrix to regenerate tissues. Silver nanoparticles is an important nanotechnology material with many utilities in some areas such as medicine, biology and chemistry. The present study focused on the synthesis of silver nanoparticles (AgNPs) stabilized with type I collagen (AgNPcol) to build a nanomaterial with biological utility. Three formulations of AgNPcol were physicochemical characterized, antibacterial activity in vitro and cell viability assays were analyzed. AgNPcol was characterized by means of the following: ultraviolet-visible spectroscopy, dynamic light scattering analysis, Fourier transform infrared spectroscopy, atomic absorption analysis, transmission electron microscopy and of X-ray diffraction analysis.ResultsAll AgNPcol showed spherical and positive zeta potential. The AgNPcol at a molar ratio of 1:6 showed better characteristics, smaller hydrodynamic diameter (64.34±16.05) and polydispersity index (0.40±0.05), and higher absorbance and silver reduction efficiency (0.645 mM), when compared with the particles prepared in other mixing ratios. Furthermore, these particles showed antimicrobial activity against both Staphylococcus aureus and Escherichia coli and no toxicity to the cells at the examined concentrations.ConclusionsThe resulted particles exhibited favorable characteristics, including the spherical shape, diameter between 64.34 nm and 81.76 nm, positive zeta potential, antibacterial activity, and non-toxicity to the tested cells (OSCC).

【 授权许可】

Unknown   
© Cardoso et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.