Assessment of ionic and anionic surfactants effect on demineralized osteochondral tissue

International Conference on Innovative Research - ICIR EUROINVENT 2019

Cobzac, V.^1 ; Veretiuc, L.^2 ; Jian, M.^1 ; Nacu, V.^1

State Medical and Pharmaceutical University nicolae Testemitanu, Bd. Stefan cel Mare 165, Chisinau

MD2004, Moldova^1

Grigore T. Popa University of Medicine and Pharmacy, 9-13 Kogalniceanu Street, Iasi

700454, Romania^2

关键词: Cell viability;    Cytotoxicity test;    Decellularized;    Decellularized tissues;    Elasticity moduli;    Enzymatic Degradation;    Osteochondral tissues;    Swelling test;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/572/1/012084/pdf
DOI  :  10.1088/1757-899X/572/1/012084

来源: IOP

【 摘 要 】

After demineralization the rabbit distal femoral osteochondral tissues were decellularized, separately with SDS and Triton X-100 for 24, 48 and 72 hours, at concentrations of 2%, 1% and 0.5%, respectively. The greatest DNA removal was achieved with Triton X-100 solutions. Cytotoxicity tests with CSM and chondrocytes have shown good and very good results, but a gradual decrease in cell viability related to the duration of treatment with surfactants compared to the control was registered. The same trend was observed in the cells population test after 7 days, while there was no difference at the 14th day. It was also determined that samples decellularized with SDS have a higher resistance to enzymatic degradation than the control and the decellularized tissue with Triton X-100. The swelling test and elasticity modulus measurements did not show values dependent of the surfactant nature.