Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin

Journal of Nanobiotechnology

Research

Ying Kong¹  Jeffrey D Cirillo¹  Yan Zhou²  Subrata Kundu³  Hong Liang⁴ 

[1] Department of Microbial and Molecular Pathogenesis, Texas A&M Health Science Center, 77807, Bryan, TX, USA;Materials Science and Engineering, Texas A&M University, College Station, 77843, TX, USA;Materials Science and Engineering, Texas A&M University, College Station, 77843, TX, USA;Department of Mechanical Engineering, Texas A&M University, College Station, 77843, TX, USA;ECMS Division, Central Electrochemical Research Institute, 630006, Tamilnadu, India;Materials Science and Engineering, Texas A&M University, College Station, 77843, TX, USA;Department of Mechanical Engineering, Texas A&M University, College Station, 77843, TX, USA;Materials Science and Engineering, Texas A&M University, College Station, 77843, TX, USA;

关键词: Antibacterial effect;    Gold;    Silver;    Nanoparticle;    BCG;   

DOI  :  10.1186/1477-3155-10-19

 received in 2012-01-12, accepted in 2012-04-16,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundDiseases such as tuberculosis (TB) have always had a large impact on human health. Bacillus Calmette-Guérin (BCG) is used as a surrogate for TB during the development of anti-TB drugs. Nanoparticles (NPs) have attracted great interest in drug development. The purpose of this study was to examine the potential of NPs as anti-TB compounds by studying the interacting mechanisms between NPs and bacteria.ResultsWe investigated effects of gold and silver NPs on BCG and Escherichia coli. Experimentally, particle size and shape were characterized using transmission electron microscopy (TEM). Different concentrations of NPs were applied in bacterial culture. The growth of E. coli was monitored through colony forming units (CFU). The mechanism of interaction between NPs and bacteria was analyzed through bacterial thin sections followed by TEM and scanning electron microscopy. Antibacterial effects on BCG were observed by recording fluorescent protein expression levels.ConclusionsThe results suggest NPs have potential applications as anti-TB compounds. The antibacterial effects and mechanism of action for NPs were dependent upon composition and surface modifications.

【 授权许可】

Unknown   
© Zhou et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.