Down-regulation of progesterone receptor membrane component 1 (PGRMC1) in peripheral nucleated blood cells associated with premature ovarian failure (POF) and polycystic ovary syndrome (PCOS)

Reproductive Biology and Endocrinology

Research

Niklas Dahl¹  Teresia Karlsson¹  Jens Schuster¹  Inger Sundström Poromaa²  Per-Olof Karlström³ 

[1] Department of Genetics and Pathology, Uppsala University, 751 85, Uppsala, Sweden;Department of Women's and Children's Health, Uppsala University, 751 85, Uppsala, Sweden;Department of Women's and Children's Health, Uppsala University, 751 85, Uppsala, Sweden;Department of Clinical Science, Karolinska University Hospital, 141 86, Stockholm, Sweden;

关键词: Menstrual Cycle;    Polycystic Ovary Syndrome;    PCOS Patient;    Premature Ovarian Failure;    Early Follicular Phase;   

DOI  :  10.1186/1477-7827-8-58

 received in 2010-03-08, accepted in 2010-06-10,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundProgesterone receptor membrane component 1 (PGRMC1) is a member of a progesterone-binding complex implicated in female reproduction. We aimed i) to determine the natural expression of PGRMC1 in peripheral nucleated blood cells throughout the menstrual cycle and ii) to investigate any association between PGRMC1 levels in leukocytes and conditions characterized by reduced fertility.MethodsWe analyzed PGRMC1 expression in peripheral leukocytes from 15 healthy cycling women over four weeks. Additionally, we determined PGRMC1 levels in samples from patients with premature ovarian failure (POF) and polycystic ovary syndrome (PCOS) as well as in healthy postmenopausal women and male controls. The levels of PGRMC1 protein in nucleated peripheral blood cells were quantified by Western blot analysis.ResultsPGRMC1 levels did not vary significantly throughout the menstrual cycle. We observed a significant down-regulation of PGRMC1 in postmenopausal women and in patients with premature ovarian failure (POF) and polycystic ovary syndrome (PCOS) when compared to early follicular phase of healthy women.ConclusionThis study suggests that reduced levels of PGRMC1 in peripheral leukocytes are associated with perturbed ovulatory function.

【 授权许可】

CC BY   
© Schuster et al; licensee BioMed Central Ltd. 2010