Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages

BMC Veterinary Research

Research Article

Jan Matiasovic¹  Jana Prodelalova¹  Martin Faldyna¹  Jan Gebauer²  Nada Zemankova²  Katarina Chlebova² 

[1] Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic;Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic;Faculty of Science, Masaryk University, Brno, Czech Republic;

关键词: Inflammatory cytokines;    LPS;    NFκB;    NIK;    TLR4;   

DOI  :  10.1186/s12917-016-0878-2

 received in 2016-05-23, accepted in 2016-11-02,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLactoferrin (LF) is an 80 kDa glycoprotein which is known for its effects against bacteria, viruses and other pathogens. It also has a high potential in nutrition therapy and welfare of people and a variety of animals, including piglets. The ability to bind lipopolysaccharide (LPS) is one of the described anti-inflammatory mechanisms of LF. Previous studies suggested that cells can be stimulated even by LPS-free LF. Therefore, the aim of our study was to bring additional information about this possibility. Porcine monocyte derived macrophages (MDMF) and human embryonic kidney (HEK) cells were stimulated with unpurified LF in complex with LPS and with purified LF without bound LPS.ResultsBoth cell types were stimulated with unpurified as well as purified LF. On the other hand, neither HEK0 cells not expressing any TLR nor HEK4a cells transfected with TLR4 produced any pro-inflammatory cytokine transcripts after stimulation with purified LF. This suggests that purified LF without LPS stimulates cells via another receptor than TLR4. An alternative, TLR4-independent, pathway was further confirmed by analyses of the NF-kappa-B-inducing kinase (NIK) activation. Western blot analyses showed NIK which activates different NFκB subunits compared to LF-LPS signaling via TLR4. Though, this confirmed an alternative pathway which is used by the purified LF free of LPS. This stimulation of MDMF led to low, but significant amounts of pro-inflammatory cytokines, which can be considered as a positive stimulation of the immune system.ConclusionOur results suggest that LF’s ability is not only to bind LPS, but LF itself may be a stimulant of pro-inflammatory pathways.

【 授权许可】

CC BY   
© The Author(s). 2016