Engagement of CEACAM1 by Helicobacter pylori HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells

Microorganisms

Raquel Mejías-Luque¹  Youssef Hamway¹  Karin Mink¹  Markus Gerhard¹  Anna Ralser¹  Karin Taxauer¹  Alisa Dietl¹  Bernhard B. Singer²  Michael Vieth³ 

[1] Institute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, 81675 Munich, Germany;Institute of Anatomy, Medical Faculty, University of Duisburg-Essen, 45147 Essen, Germany;Institute of Pathology, Friedrich-Alexander University Erlangen-Nuremberg, Klinikum Bayreuth, 95445 Bayreuth, Germany;

关键词: non-canonical NF-ĸB;    NIK;    p52;    H. pylori;    HopQ;    CEACAM;   

DOI  :  10.3390/microorganisms9081748

来源: DOAJ

【 摘 要 】

The gastric pathogen Helicobacter pylori infects half of the world’s population and is a major risk factor for gastric cancer development. In order to attach to human gastric epithelial cells and inject the oncoprotein CagA into host cells, H. pylori utilizes the outer membrane protein HopQ that binds to the cell surface protein CEACAM, which can be expressed on the gastric mucosa. Once bound, H. pylori activates a number of signaling pathways, including canonical and non-canonical NF-κB. We investigated whether HopQ–CEACAM interaction is involved in activating the non-canonical NF-κB signaling pathway. Different gastric cancer cells were infected with the H. pylori wild type, or HopQ mutant strains, and the activation of non-canonical NF-κB was related to CEACAM expression levels. The correlation between CEACAM levels and the activation of non-canonical NF-κB was confirmed in human gastric tissue samples. Taken together, our findings show that the HopQ–CEACAM interaction is important for activation of the non-canonical NF-κB pathway in gastric epithelial cells.

【 授权许可】

Unknown