Mycobacterium tuberculosis Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen

BMC Microbiology

Research Article

Manuel E Patarroyo¹  Diana P Cifuentes²  Hernando Curtidor²  Marisol Ocampo²  Manuel A Patarroyo²  Martha Forero²  Magnolia Vanegas³ 

[1] Fundación Instituto de Inmunología de Colombia, Carrera 50 No., 26-20, Bogotá, Colombia;Universidad Nacional de Colombia, Carrera 45 No., 26-85, Bogotá, Colombia;Fundación Instituto de Inmunología de Colombia, Carrera 50 No., 26-20, Bogotá, Colombia;Universidad del Rosario, Calle 63 D No., 24-31, Bogotá, Colombia;Fundación Instituto de Inmunología de Colombia, Carrera 50 No., 26-20, Bogotá, Colombia;Universidad del Rosario, Calle 63 D No., 24-31, Bogotá, Colombia;Universidad Nacional de Colombia, Carrera 45 No., 26-85, Bogotá, Colombia;

关键词: Tuberculosis;    A549 Cell;    U937 Cell;    Latex Bead;    Tuberculosis H37Rv;   

DOI  :  10.1186/1471-2180-10-109

 received in 2009-09-24, accepted in 2010-04-13,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundTo date, the function of many hypothetical membrane proteins of Mycobacterium tuberculosis is still unknown and their involvement in pathogen-host interactions has not been yet clearly defined. In this study, the biological activity of peptides derived from the hypothetical membrane protein Rv0679c of M. tuberculosis and their involvement in pathogen-host interactions was assessed. Transcription of the Rv0679c gene was studied in 26 Mycobacterium spp. Strains. Antibodies raised against putative B-cell epitopes of Rv0679c were used in Western blot and immunoelectron microscopy assays. Synthetic peptides spanning the entire length of the protein were tested for their ability to bind to A549 and U937 cells. High-activity binding peptides (HABPs) identified in Rv0679c were tested for their ability to inhibit mycobacterial invasion into cells.ResultsThe gene encoding Rv0679c was detected in all strains of the M. tuberculosis complex (MTC), but was only transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra and M. africanum. Anti-Rv0679c antibodies specifically recognized the protein in M. tuberculosis H37Rv sonicate and showed its localization on mycobacterial surface. Four HABPs inhibited invasion of M. tuberculosis to target cells by up to 75%.ConclusionsThe results indicate that Rv0679c HABPs and in particular HABP 30979 could be playing an important role during M. tuberculosis invasion of host cells, and therefore could be interesting research targets for studies aimed at developing strategies to control tuberculosis.

【 授权许可】

Unknown   
© Cifuentes et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.